The effect of anti-CD28 on the CD3-AK proliferation and tumoricidal activity

Objective

The aim of this study was to costimulate the CD3-AK cells with anti-CD28 monoclonal antibody (mAb), observe the effect of cell proliferation and cytotoxicity and explore the regulatory role of CD28 mAb on the CD3-AK tumoricidal activity.

Methods

To prepare CD3-AK cells, observe the number and morphology of the activated T cells with the inverted microscope and detect the inhibitory rate of the myeloma cells by methylthiazolyldiphenyl-tetrazolium bromide (MTT).

Results

Adding the anti-CD28 mAb to the CD3-AK cells, the number of cells increased significantly (P < 0.05) and the cytotoxic activity of the cells was significantly higher (P < 0.05).

Conclusion

The addition of anti-CD28 mAb has a strong synergistic effect, which can effectively enhance the tumoricidal activity of CD3-AK.