肝动脉重建的小鼠原位肝移植模型建立

目的 建立稳定的肝动脉重建的小鼠原位肝移植模型.方法 以"双袖套法"小鼠肝移植为基础,将带腹主动脉的供肝肝动脉与受体腹主动脉行端侧吻合.共完成小鼠原位肝移植70例,并观察术后24 h、1周、1个月受体存活率,术后1个月肝功能及肝组织病理变化.同时设立假手术组对照.结果 受体术后24 h、1周、1个月存活率分别为94.3%、91.4%、85.7%.术后1个月肝功能ALT:(39.20±2.09)U/L AST:(75.60±3.24)U/L.与对照组比较,差异无统计学意义(P>0.05).病理显示肝组织结构良好.结论 该方法可稳定地建立肝动脉重建的小鼠原位肝移植模型.

Surgical experience of arterialized orthotopic liver transplantation model in mice

Objective To establish the arterialized liver transplantation model in mice. Methods On the basis of "two cuff" orthotopic liver transplantation in mice, graft aorta end-to-side anastomosis to recipient aorta was applied to reconstruct hepatic artery. Twenty-four h-, 1 week-, 1 month-postoperative survival rate, hepatic function, and pathological changes were observed. Sham operation group was set up. Results 94.3% ,91.4% and 85.7% of recipients survived after 48 h,one week and one month ,respec-tively. The liver functioned normally after one month postoperatively. Pathologically, the hepatic tissue had good structure. Conclusion It is an ideal mothods to establish the arterialized orthotopic liver transplanta-tion model in mice.