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Detection of Mycoplasma pneumoniae by two polymerase chain reactions and role of Mycoplasma pneumoniae in pediatric community–acquired lower respiratory tract infections |
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| Authors: | Surinder Kumar Pradeep Kumar Bharti CP Baveja Mukta Mantan Sanjeev R Saigal Indu Bala Garg |
| Institution: | 1. Department of Microbiology, Maulana Azad Medical College, New Delhi, 110002, India;2. Department of Pediatrics, Maulana Azad Medical College, New Delhi, 110002, India;1. Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India;2. Department of General Surgery, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India;3. Department of Surgical Oncology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India;4. Department of Surgical Gastroenterology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India;1. Department of Microbiology, Velammal Medical College Hospital and Research Institute, Madurai, India;2. Department of Microbiology, AIIMS, Bibinagar, India;1. Department of Microbiology, All India Institute of Medical Sciences, New Delhi, 110029, India;2. Department of Pulmonary Medicine and Sleep Disorders, All India Institute of Medical Sciences, New Delhi, 110029, India;3. Department of Laboratory Medicine, Jai Prakash Narayan Apex Trauma Centre, All India Institute of Medical Sciences, New Delhi, 110029, India;1. Department of Microbiology, AIIMS, New Delhi, India;2. Department of Cardiothoracic and Vascular Surgery, AIIMS, New Delhi, India |
| Abstract: | PurposeThe study was conducted to evaluate the role of Mycoplasma pneumoniae (M. pneumoniae) in children with community-acquired lower respiratory tract infections (LRTIs).MethodsSeventy five children aged 2 months ?12 years with community-acquired LRTIs were investigated for M. pneumoniae etiology employing paired serum samples to assay M. pneumoniae antibodies. Nasopharyngeal aspirates were obtained for the detection of M. pneumoniae by using polymerase chain reaction(PCR) and nested PCR.ResultsM. pneumoniae infection was positive in 24(85.71%) children aged <5 years and 4 (14.29%) ?≥ ?5–12 years and the difference was statistically insignificant (P ?= ?0.18). Difference in prevalence of M. pneumoniae infection across male and female groups was statistically insignificant (P ?= ?0.69). Clinical and radiological profiles across M. pneumoniae positive and negative cases were comparable except bronchopneumonia which was statistically significant (P ?= ?0.04). Serological evidence of M. pneumoniae infection was observed in 26(33%); PCR was positive in 9 (12%) and nested PCR in 10 (13.33%) children. Together, serology, PCR and nested PCR diagnosed M. pneumoniae infection in 28(37.33%) patients. Sensitivity of serology was 77.78%: specificity 68.18%; positive predictive value 25.00% and negative predictive value at 95.74%.ConclusionsSerological and molecular methods in combination is useful for detection of M. pneumoniae. Our data underline the role of M. pneumoniae in community-acquired LRTIs in children of all ages. |
| Keywords: | Children Lower respiratory tract infections Polymerase chain reaction Serology |
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