护心通络方通过干预CETP介导脂质代谢抗兔动脉粥样硬化的实验研究

目的 探究护心通络方通过调控胆固醇脂转运蛋白（CETP）抗兔动脉粥样硬化（AS）的效果及作用机制。方法 将40只雄性新西兰兔按照随机数字表法分为空白对照组、AS模型组、辛伐他汀组[0.33 mg/（kg·d）]、护心通络方组[0.34 g/（kg·d）]，每组10只。空白对照组予普通饲料喂养22周，其他3组予高脂饲料喂...

Experimental study of huxintongluo decoction on rabbit atherosclerosis by intervening CETP mediated lipid metabolism

Objective To study the effect and mechanism of huxintongluo decoction on rabbit atherosclerosis (AS) by regulating cholesterol lipid transporter protein (CETP). Methods Forty male New Zealand rabbits were divided into the blank control group, the AS model group, the simvastatin group [0.33 mg/(kg·d)] and the huxintongluo group [0.34 g/(kg·d)] according to random number table method, with 10 rabbits in each group. The blank control group was fed ordinary diet for 22 weeks. The other three groups were fed with high fat diet for 14 weeks, and then different drug intervention was performed in groups for 8 weeks. Serum levels of high density lipoprotein (HDL-C), low density lipoprotein (LDL-C), total cholesterol (TC) and triglyceride (TG) were detected before the experiment, 14 and 22 weeks after administration. The animals were sacrificed at the end of the 22-week, the thoracic aorta was separated, and the pathological changes were observed by hematoxyl-eosin (HE) staining. The expression levels of ABCA1, apoA-I and LCAT in liver tissues were detected by fluorescence quantitative PCR and Western blot assay. Results There were no significant differences in serum levels of TC, TG, HDL-C and LDL-C before the experiment between the four groups (P>0.05). At week 14 of experiment, compared with the blank control group, serum levels of TC, TG and HDL-C were significantly increased in the other three groups, while the serum level of LDL-C was significantly decreased (P<0.05). At week 22 of experiment, compared with the AS model group, serum levels of TC, TG and LDL-C were significantly decreased in the other three groups, while serum level of HDL-C was significantly increased (P<0.05). Compared with the blank control group, the serum level of CETP increased in the AS model group. Compared with the AS model group, the serum level of CETP decreased in the simvastatin group and the huxintongluo group. Compared with the blank control group, ABCA1, apoA-I and LCAT mRNA and protein levels in liver tissues were decreased in the AS model group. Compared with the AS model group, mRNA and protein levels of ABCA1, apoA-I and LCAT were increased in the simvastatin group and the Huxintongluo group (P<0.05). Conclusion Huxintongluo decoction can activate ABCA1/apoA-I/LCAT pathway by reducing CETP level, ultimately improve HDL-C level and improve atherosclerosis in rabbits.