Comparison of a chemiluminescence immunoassay and an enzyme immunoassay for detection of IgM antibodies against measles,mumps, rubella,cytomegalovirus (CMV), Epstein Barr virus (EBV), and human herpes virus (HHV) -1 and -2 infections |
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| Authors: | Runal John Steve Shoba Mammen Kavitha Selvaraj Bijesh Yadav Asha Mary Abraham |
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| Affiliation: | 1. Department of Clinical Virology, Christian Medical College, Vellore, Tamil Nadu, India;2. Department of Biostatistics, Christian Medical College, Vellore, Tamil Nadu, India;1. Department of Biotechnology, Mizoram University, Aizawl 796004, Mizoram, India;2. Department of Microbiology, Synod Hospital, Durtlang, Aizawl 796025, Mizoram, India;3. Department of Health and Family Welfare, Directorate of Health Services, Aizawl 796009, Mizoram, India;4. Directorate of Health Services, National Health Mission, Aizawl 796009, Mizoram, India;5. CMO, Health & Family Welfare Department, Mamit 796441, Mizoram, India;6. Department of Microbiology, Zoram Medical College, Falkawn 796005, Mizoram, India;7. Cellular Immunology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India;1. Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences (SGPGIMS), Lucknow, India;2. Department of Microbiology, GIPMER, Delhi, India;1. Hacettepe University Faculty of Medicine, Department of Medical Microbiology, Ankara, Turkey;2. Hacettepe University Faculty of Medicine, Department of Biostatistics, Ankara, Turkey;1. YRG Centre for AIDS Research and Education, Chennai, India;2. Vellore Institute of Technology, Vellore, India;3. Johns Hopkins University School of Medicine, Department of Medicine, Baltimore, MD, USA |
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| Abstract: | PurposeOutbreaks of vaccine-preventable viral diseases have been increasingly reported globally over the past few years. The burden of congenital viral infections, their impact on physical and mental development and the resulting economic loss to the family and the community are also well known. IgM antibody detection has been convenient in the diagnosis of acute viral infections, particularly in settings with limited resources where molecular tests are not feasible.MethodsThis is a comparative study between a chemiluminescence immunoassay (Liaison, DiaSorin, Saluggia, Italy) and an enzyme linked immunosorbent assay (ELISA) (Euroimmun, Lubeck, Germany) for the detection of IgM antibody against measles, mumps, rubella, CMV, EBV and HHV-1 and -2 viruses using a total of 345 samples. Results are expressed as agreement using kappa statistics.ResultsIn this study, CLIA is perfectly comparable to ELISA for the detection of IgM antibodies against measles (0.86) and mumps (0.92) with a moderate agreement for rubella (0.52), CMV (0.57), EBV (0.50), and HHV-1 and -2 (0.47) assays. However, a PABAK (prevalence-adjusted bias-adjusted kappa) showed improved agreement for rubella (0.64), CMV (0.65), EBV (0.60), and HHV-1 and -2 (0.88) assays.ConclusionsIgM antibody assays (CLIA and ELISA) against measles and mumps virus can be comparably used depending on the laboratory setup, throughput and expertise. |
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| Keywords: | Chemiluminescence immunoassay (CLIA) Enzyme linked immunosorbent assay (ELISA) Measles Mumps Rubella |
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