CD1a标记树突细胞在声门型喉鳞癌中的表达及其临床意义

背景与目的:树突细胞(dendriticcell,DC)是最重要的抗原呈递细胞(antigenpresentingcell,APC),而CD1a作为未成熟DC的标记物,在肿瘤的发生及发展过程中有一定的影响。本文旨在探讨CD1a DC与声门型喉癌病理分级、T分期及局部复发的关系,从而间接了解它与预后的关系。方法:收集111例有完整病理资料及临床随诊资料的声门型喉鳞癌病例,并取17例非肿瘤组织做对照。用免疫组化技术检测CD1a DC在癌组织中的表达,分析癌组织中CD1a DC在不同病理分级、T分期及局部复发组织中的表达,间接了解其与声门型喉鳞癌预后的关系。结果:111例声门型喉鳞癌病例中,CD1a阳性率为59.46%(66/111);高分化组阳性率为71.43%(55/77),中低分化组阳性率为28.57%(11/34);T1 T2组阳性率为67.16%(45/67),T3 T4组阳性率为47.73%(21/44)。局部复发组阳性率为42.86%(12/28),局部无复发组阳性率为65.06%(54/83)。17例非肿瘤组织中CD1a均为阴性。结论:声门型喉鳞癌中,肿瘤细胞分化越差,T分期越高,则CD1a标记树突细胞阳性率越低。局部复发者CD1a DC阳性率低。CD1a可作为预测声门型喉鳞癌预后的参考指标之一。

Expression and clinical significance of CD1a+ dendritic cells in glottic squamous cell carcinoma

BACKGROUND & OBJECTIVE: Dendritic cells (DCs) are the most important antigen-presenting cells. CD1a, a marker of immature DCs, plays a role in tumorigenesis. This study was designed to explore the correlation of CD1a+ DCs to pathologic degree, T stage, local recurrence, and prognosis of glottic squamous cell carcinoma. METHODS: Clinical and follow-up data of 111 patients with glottic squamous cell carcinoma were analyzed retrospectively. The expression of CD1a+ DCs in the 111 specimens of glottic squamous cell carcinoma and 17 specimens of non-tumor tissue was detected by immunohistochemistry. Correlations of CD1a+ DCs to pathologic degree, T stage, local recurrence, and prognosis of glottic squamous cell carcinoma were analyzed. RESULTS: Positive rates of CD1a+ DCs were 59.46% in glottic squamous cell carcinoma tissues, and 0 in non-tumor tissues; it was significantly higher in well-differentiated group than in moderately- and poorly-differentiated group 71.43% (55/77) vs. 28.57% (11/34), P<0.05], higher in T1-T2 group than in T3-T4 group 67.16% (45/67) vs. 47.73% (21/44), P<0.05], and higher in non-recurrence group than in local recurrence group 65.06% (54/83) vs. 42.86% (12/28), P<0.05]. CONCLUSIONS: The lower pathologic degree and higher T stage of glottic squamous cell carcinoma, the lower expression of CD1a+ DCs. The positive rate of CD1a+ DCs is low in recurrent glottic tumor. High expression of CD1a+ DCs may indicate better prognosis of glottic squamous cell carcinoma.