| 三氧化二砷对人肺腺癌Anip973细胞内[Ca2+]i的影响 |
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| 引用本文： | 师晶玉,李宝馨,孙宏丽,谷东方,李丽,吴红,杨宝峰. 三氧化二砷对人肺腺癌Anip973细胞内[Ca2+]i的影响[J]. 哈尔滨医科大学学报, 2004, 38(2): 146-148 |
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| 作者姓名： | 师晶玉 李宝馨 孙宏丽 谷东方 李丽 吴红 杨宝峰 |
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| 作者单位： | 哈尔滨医科大学药理学教研室,黑龙江,哈尔滨150086;哈尔滨医科大学药理学教研室,黑龙江,哈尔滨150086;哈尔滨医科大学药理学教研室,黑龙江,哈尔滨150086;哈尔滨医科大学药理学教研室,黑龙江,哈尔滨150086;哈尔滨医科大学药理学教研室,黑龙江,哈尔滨150086;哈尔滨医科大学药理学教研室,黑龙江,哈尔滨150086;哈尔滨医科大学药理学教研室,黑龙江,哈尔滨150086 |
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| 摘 要： | 目的研究三氧化二砷(As2O3)对人肺腺癌Anip973细胞内游离[Ca2+]i的影响.方法培养人肺腺癌Anip973细胞,用激光扫描共聚焦显微镜(LSCM)观察As2O3对人肺腺癌Anip973细胞内游离[Ca2+]i的影响;用生长曲线法测药物对肿瘤细胞增殖的影响.结果①LSCM显示As2O3 作用后的人肺腺癌细胞Anip973细胞内[Ca2+]i显著升高(P<0.001).②As2O3 对人肺腺癌细胞株Anip973细胞内[Ca2+]i升高作用具有剂量依赖性.③As2O3 能明显抑制人肺腺癌Anip973细胞的增殖.结论 As2O3 可能是通过升高人肺腺癌细胞内[Ca2+]i 来启动细胞凋亡机制,诱导肺癌细胞凋亡,从而抑制其增殖.
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| 关 键 词： | 三氧化二砷 细胞内游离钙 激光扫描共聚焦显微镜 生长曲线 |
| 文章编号： | 1000-1905(2004)02-0146-03 |
| 修稿时间： | 2003-09-15 |
Variation of [Ca2+]i induced by arsenic trioxide in human lung adenocarcinoma cell line |
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| SHI Jing yu,LI Bao xin,SUN Hong li,et al. Variation of [Ca2+]i induced by arsenic trioxide in human lung adenocarcinoma cell line[J]. Journal of Harbin Medical University, 2004, 38(2): 146-148 |
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| Authors: | SHI Jing yu LI Bao xin SUN Hong li et al |
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| Abstract: | Objective To explore the effect of arsenic trioxide (As 2O 3) on intracellular free calcium concentration.Methods A single cell [Ca 2+ ]i was measured by laser scanning confocal microscope using Ca 2+ sensitive dye Fluo 3 /AM as a new calcium fluorescent probe; The antiproliferation activities of As 2O 3 on Anip973 cells were evaluated by means of continuous growth curve.Results As 2O 3 could obviously increase the concentration of intracellular free calcium in dose dependent manner.Conclusion The antitumor mechanism of arsenic trioxide may be that it can induce Anip973 cell apoptosis through increasing [Ca 2+ ]i level; As 2O 3 can inhibit the proliferation of Anip973 cells significantly. |
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| Keywords: | arsenic trioxide intracellular free calcium laser scanning confocal microscope cell growth curve |
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