用PCR技术及常规病毒学检测诊断呼吸道合胞病毒感染的方法学比较

分别采用病毒分离法、免疫荧光法及NestedPCR、RT－PCR十核酸探针杂交实验对78例呼吸性疾病患儿鼻腔抽吸物标本中呼吸道合胞病毒（RSV）的感染水平进行了检测。结果表明，在这4种检测方法中，以NestedPCR（巢式PCR）法最为敏感、稳定，RSV感染的阳性率为29·49％，且省时，准确。

Comparison of PCR and Standard Virology in Diagnosis of Respiratory Syncytial Virus(RSV) Infection in Children with Respiratory Diseases

By two standard virologic methods(virus culture, Immunofluorescence) adn two PCR methods(Nested PCR, RT-PCR Probe hybridization) the rate of RSV infection in 78 nasal aspirates from the children with respiratory diseases was examined, and comparison between standard virologic methods and PCR was conducted. The results showed that Nested PCR was both sensitive and specific in diagnosis of RSV infection, and moderately advance over conventional virologic methods. In addition, the one of major advantages of PCR over virus culture is in that it is fast.