CTGF在兔眼深层巩膜切除术中的表达

目的:探讨结缔组织生长因子(connective tissue growthfactor,CTGF)在兔眼深层巩膜切除术(deep sclerectomy,DS)中的表达。方法:制备人去内皮冻干辐照脐带静脉管(HUV);选取28只大耳白兔,其中24只按自身对照原则,双眼均行深层巩膜切除术,随机选取一眼于术中植入HUV,为植入组;另一眼不植入,为未植入组。分别于术后3,7,14d各处死8只;另4只8眼做正常对照组。应用半定量PCR检测兔眼深层巩膜组织中CTGF mRNA的表达,免疫组织化学检测CTGF蛋白的表达。结果:植入组3d后,CTGF mRNA和蛋白表达开始升高,至7d表达水平达到峰值,14d时表达水平开始下降,明显高于对照组(P<0.01),未植入组中不同时间点表达水平变化与植入组类似。植入组与未植入组比较无统计学意义(P>0.05)。结论:HUV在兔眼DS中没有明显的抗纤维增殖的作用,其瘢痕增殖的过程与正常瘢痕形成的过程是一致的。其维持减压室作用机制可能是机械引流的作用结果;HUV作为一种植入物,并未刺激局部的瘢痕过度形成。

Expression of CTGF in rabbits deep sclerectomy surgery

·AIM:To investigate the expression of connective tissue growth factor（CTGF）in rabbits deep sclerectomy（DS）surgery.·METHODS:Human umbilical vein（HUV）was treated with freeze-drying for 6 hours and ⁶⁰Co200cGy irradiation for 30 seconds.The samples were stored in RT.28 rabbits were used,24 of which were performed DS surgery in both eyes.Then one eye was randomly selected to plant HUV according to self-control rule,which composed experimental group.The other eye of the 24 rabbits composed control group.8 rabbits were sacrificed 3,7 and 14 days after operation,respectively.The 8 eyes of the other 4 rabbits were used as normal control.Semi-quantitative RT-PCR and immunohistochemistry were used to assess the expression of CTGF mRNA and protein of deep sclera.·RESULTS:The levels of CTGFmRNA and protein expression in the normal control tissue were lower,but at day 3 they increased more quickly than the control and rapidly peaked at day 7,then decreased at day 14（P<0.01）;there were no significant differences at same time points between experimental group and control group（P>0.05）.·CONCLUSION:These indicated that DS surgery with huaman freeze-dried allograft umbilical vein implant is safe and effective.It has no evident effect of anti-fiber proliferation.The mechanism for its maintanence of decompression chamber may be the result of mechanical water spreading.·