兔眼小梁切除术中巩膜瓣下植入陈旧生物羊膜的病理变化

目的:评价青光眼滤过手术中应用陈旧生物羊膜抗纤维增殖作用。方法:采取随机对照动物实验研究方法,选日本大耳兔30只,共60眼,采用随机数字表法将其分为两组。两组中右眼(30眼)均行小梁切除术,术中巩膜瓣层间植入陈旧生物羊膜,组A左眼(15眼)行小梁切除术联合应用干扰素α-2b,组B左眼(15眼)仅行单纯小梁切除术。并于术后3,7,14,30,60d分别处死两组中各3只动物,摘除眼球作病理切片,观察羊膜降解时间、成纤维细胞和炎症细胞计数。结果:羊膜组早期即可见大量成纤维细胞增殖,成纤维细胞处于功能活跃状态。羊膜组炎性细胞数目、成纤维细胞数目,术后3,7,14d均高于其它两组。羊膜大致在14～21d内降解。结论:兔眼小梁切除术中应用陈旧的生物羊膜诱发了兔眼的免疫排斥反应。

Pathological results of obsolete amniotic membrane for glaucoma filtering operation in rabbits

·AIM:To investigate the anti-fibrosis effect of obsolete amniotic membrane for glaucoma filtering operation in rabbits.·METHODS:Trabeculectomy was performed in 60 eyes of 30 rabbits which were divided into 2 groups.In two groups,right eyes were treated with the obsolete amniotic transplantation under sclerotic flap.In group A,the left eyes were performed trabeculectomy using interferon α-2b.In group B the left eyes played the role of control which had surgery only.3 rabbits which were selected randomly in the two groups were killed 3,7,14,30,60 days after operation and eyeballs were enucleated to observe the time of decomposition of the amniotic membrane in pathologic tissue slice,the number of fibroblast and inflammatory cells were counted with computer and their difference was compared.·RESULTS:The number of inflammatory and fibroblast cells was higher than the other groups in the amniotic membrane transplantation group 3,7,14 days after operation,which showed significant difference.The decomposed time of the amniotic membrane was about 14-21 days.·CONCLUSION:Amniotic membrane immunologic rejection reaction was induced through obsolete amniotic membrane transplantion for glaucoma filtering operation in rabbits.·