葛根素对糖尿病视网膜病变中Bcl-2表达的影响

目的:通过分析凋亡细胞及凋亡通路中的关键因子Bcl-2表达的变化,阐明葛根素抗凋亡的作用和机制。方法:取成年健康大鼠60只,随机分为空白对照组、糖尿病(DM)组和DM葛根素干预组,ip链脲佐菌素建立DM大鼠视网膜病变模型,3mo时处死大鼠观察各组视网膜组织病理变化,并应用RT-PCR对Bcl-2进行定量分析。结果:DM大鼠HE染色可见神经节细胞减少,内外颗粒层平均光密度减低,有空泡形成,细胞排列紊乱稀疏。葛根素干预组细胞结构好转,未见空泡。RT-PCR可见Bcl-2在葛根素干预组表达明显高于正常组和DM组(P<0.05)。结论:葛根素可改善视网膜组织病理学变化,并增强Bcl-2的表达。

Effect of puerarin on expression of Bcl-2 in the retina of diabetic rats and the possible mechanism

·AIM:To investigate the effect of puerarin on apoptosis expression of Bcl-2 in the retina of diabetic rats and the possible mechanism of puerarin.·METHODS:Totally 60 male Wister rats were selected and divided at random into 3 groups:normal group,diabetic group and diabetic with puerarin injection group.The diabetic retinopathy rats model was established by injecting streptozotocin(STZ).The pathogenic changes of diabetic retinopathy were observed and a quantitative analysis was given to Bcl-2 with RT-PCR at month 1,3 and 5,the data were analyzed with software SPSS 13.0.·RESULTS:Bcl-2 expressed in the retina of the normal rats.When at month 3,the expression of Bcl-2 was remarkably increased in diabetic group by puerarin injecting comparing with diabetic group(P<0.05),while at month 5,the expression of Bcl-2 was increased in the two groups.The pathologic damage in the retina of diabetic groups by puerarin injecting was slighter than diabetic groups.·CONCLUSION:Puerarin can alleviate the retinal pathological damage of diabetic rats.At month 3,Bcl-2 is expressed higher in the diabetic model by puerarin injecting than the diabetic group.Puerarin can increase the process of diabetic retinopathy by restraining the expression of Bcl-2.Consequently,puerarin will probably become the effective medicine to cure diabetic retinopathy.·