| 染料木素对体外培养的牛眼小梁细胞[Ca2+]i的影响 |
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| 引用本文： | 陆慧琴,马强,张德秀,梁厚成. 染料木素对体外培养的牛眼小梁细胞[Ca2+]i的影响[J]. 国际眼科杂志, 2011, 11(6): 964-965. DOI: 10.3969/j.issn.1672-5123.2011.06.008 |
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| 作者姓名： | 陆慧琴 马强 张德秀 梁厚成 |
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| 作者单位： | 1. 中国陕西省西安市第一医院眼科,710002
2. 西安交通大学第一医院眼科,中国陕西省西安市,710061 |
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| 摘 要： | 目的:在激光扫描共聚焦显微镜(laserscanning confocal microscopy,LSCM)下测定小梁细胞经染料木素(genistein,Gen)作用后钙离子浓度([Ca2+]i)的变化。方法:运用组织块培养法获得三代牛眼小梁细胞,Fulo-3/AM负载后,于LSCM下动态扫描不同浓度Gen作用后胞内荧光强度变化,得出[Ca2+]i值。结果:10-6,10-5,10-4mol/LGen可使小梁细胞[Ca2+]i呈剂量依赖性降低,其中10-5mol/LGen可使小梁细胞[Ca2+]i由328.62±16.77nmol/L减少至309.69±16.57nmol/L(n=8,P<0.05)。结论:Gen可通过松弛小梁网而致房水流出阻力减小、眼压下降。
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| 关 键 词： | 小梁细胞 染料木素 胞内钙离子浓度 激光扫描共聚焦显微镜 |
Influnence of genistein on [Ca2+]i in cultured bovine trabecular meshwork cells in vitro |
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| Hui-Qin Lu,Qiang M,De-Xiu Zhang and Hou-Cheng Liang. Influnence of genistein on [Ca2+]i in cultured bovine trabecular meshwork cells in vitro[J]. International Eye Science, 2011, 11(6): 964-965. DOI: 10.3969/j.issn.1672-5123.2011.06.008 |
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| Authors: | Hui-Qin Lu Qiang M De-Xiu Zhang Hou-Cheng Liang |
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| Affiliation: | Department of Ophthalmology,First Hospital of Xi-an, Xi-an 710002, Shaanxi Province, China;;Department of Ophthalmology,First Hospital of Xi-an, Xi-an 710002, Shaanxi Province, China;;Department of Ophthalmology, the First Hospital of Xi-an Jiaotong University, Xi-an 710061, Shaanxi Province, China;Department of Ophthalmology,First Hospital of Xi-an, Xi-an 710002, Shaanxi Province, China; |
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| Abstract: | AIM:To determine the change of intracellular free Ca2+ in trabecular meshwork cells (TMC) under the influence of genistein(Gen) through laser scanning confocal microscopy(LSCM).METHODS: TMC were obtained through the method of tissue cultured. Loaded with Fluo-3/AM, the value of [Ca2+]i was obtained by dynamically scanning changes of intracellular fluorescent intensity after application of genistein with different density.RESULTS:The application of genistein (10-6,10-5,10-4mol/L, final concentration) led to an decrease in [Ca2+]i and especially 10-5mol/L genistein led to a significant decrease in [Ca2+]i: from 328.62±16.77 to 309.69±16.57 (n=8,P<0.05). CONCLUSION:Genistein with a certain density may decrease the resistance to the outflow of aqueous by relaxing TMC and lead to an decrease of IOP. |
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| Keywords: | trabecular meshwork cells genistein [Ca2 ]i LSCM |
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