联用重组生长激素、膳食纤维促短肠大鼠结肠形态代偿

目的：探讨联合应用肠内营养（EN）、膳食纤维（diet fiber)和重组生长激素（recombinant growth hormone,rGH)治疗极短肠大鼠，及对短肠大鼠结肠形态学代偿的促进作用。方法：制成切除80％－85％短肠大鼠动物模型，用百普素行肠内营养，并加用diet fiber和rGH。随机分成三组：肠内营养组（EN），肠内营养＋膳食纤维组（EF），肠内营养＋膳食纤维 生长激素组（EFG），及正常大鼠对照组（CONT）。观察全身营养状况、结肠形态学、结肠上皮细胞DNA和倍体及结肠组织IGF－1和IGF－1受体mRNA含量。结果：术后第15天EFG组体重减轻明显好于EN组并于第21天体重大于术前。EFG组净氮平衡的改善明显好于EF和EN组，血浆蛋白EFG组明显高于EN组。各组间形态学差异非常显著，各实验组结肠黏膜细胞DNA含量和细胞活动周期明显高于对照组，以EFG组最高。各实验组IGF－1mRNA含量比CONT组明显增高，EFG组最高；各实验组IGF－1受体mRNA含量比CONT组明显减低，以EFG组最低。结论：1．短肠综合征时大鼠结肠发生了明显的形态和功能代偿。2．Diet fiber和rGH对短肠大鼠结肠形态学的代偿有明显促进作用，联用效果最佳。

To Promote Colonic Morphological Adaption of Short Bowel Rat by Combina-tion of Enteral Nutrition, Diet Fiber and Recombinant Growth Hormone

Objective: To study the treatment for the short bowel rats by combination of enteral nutrition,diet fiber and re-combinant growth hormone in the promotion of colonic morphological adaption. Methods: Twenty rats were resected 80~ 85% small intestine and randomly assigned to one of three groups: enteral nutrition group (EN), enteral nutrition,diet fiber group(EF),enteral nutrition, diet fiber and recombinant growth hormone(rGH) group (EFG). Another ten normal rats were controls (CONT). Nutritional state,colonic morphology, IGF - ImRNA and IGF - 1 - recetpor mRNA of the colon were investigated. Results: On POD 15, body weight of the EFG group was heavier than that of EN group( -4.3