乙二醛对小鼠抗氧化系统的影响

目的：通过观察乙二醛（glyoxal）急性染毒小鼠全血及组织中超氧化物歧化酶（superoxide dismutase SOD）谷胱甘肽过氧化物酶（glutathione peroxidase GSH-Px）、巯基含量（T-SH、NP-SH）及脂质过氧化物（Lipid Peroxidation，LPO）含量的变化，探讨乙二醛对抗氧化系统的影响及毒作用机制。方法：实验用昆明种小鼠40只，分成4组。实验组分三组，腹腔注射乙二醛水溶液，剂量分别为1．3mmol／kg（1／8 LD50）、2．6mmol／kg（1／4 LD50）及5．2mmol／kg（1／2 LD50），对照组注射等量生理盐水。急性染毒后24h，采血并取心、肝、脑及肾组织。全血及组织中GSH-Px的活力测定．采用二硫双硝基苯甲酸（DTNB）法；SOD活力的测定用亚硝酸盐法。血清及组织中的LPO（主要为MDA）含量测定，用硫代巴比妥酸（TBA）法；全血及组织中总巯基（T-SH）和非蛋白巯基（NP-SH）含量测定，用Ellmans法，蛋白巯基（PB-SH）含量可由T-SH减去NP-SH求得。结果：各染毒组脑及高剂量组肝的LPO含量升高，与对照组比较差异具有显著性（P〈0．05或P〈0．01）。低剂量组肝及高剂量组脑的SOD活力增高；各剂量组的全血和肾脏及中、低剂量组肝脏GSH-Px活力下降；各剂量组全血及高剂量组脑的总巯基及蛋白巯基含量增加，高剂量组脑、肾及中、低剂量组肝脏非蛋白巯基含量下降，均与对照组相比差异显著（P〈0．05或P〈0．01）。结论：小鼠脑及肝组织对毒物诱发的自由基的高度敏感，易致脂质过氧化损伤；血、肝及肾中GSH-Px为接触乙二醛的敏感指标。

Effects of glyoxal on anti oxidative system in mice

objective ： To study the effects of glyoxal on anti-oxidative system in mice and its mechanism of toxicity through measuring the content of SOD （Superoxide Dismutase）, GSH-Px （Glutathione Peroxidase）, T-SH （Total Sulfhydryl） , NP-SH （Non- Protein Sulfhydryl） and LPO （Lipid Peroxidation） in the blood and tissue in mice treated with glyoxal. Method： This study use 40 mice of KunMing species, which are divided into 4 groups evenly on random. 1 is negative control group treated with normal saline, other 3 is experiment groups treated with glyoxal of 1.3mmol/kg （1/8 LD50） , 2. 6mmol/kg （1/4 LD50） and 5.2mmol/kg （ 1/ 2 LD50） separately. The mice were taken blood, heart, liver, brain and kidney after they were treated with glyoxal for 24 hours. The activity of GSH-Px in blood and tissue were measured by DTNB method; The content of T-SH and NP-SH in blood and tissue were measured by Ellmans, and the content of PB-SH can be got from T-SH subtracting NP-SH. The activity of SOD in blood and tissue were measured by nitrite method. The content of LPO （especially MDA） in serum and tissue was measured by TBA method. Results ： The content of LPO in various dose groups in brain and in high dose group in liver of mice is significantly higher than that of the control group （ P 〈 0. 05 or P 〈 0. 01 ）. The activity of SOD in liver and brain of high dose groups increases. The activities of GSH-Px significantly decrease in blood and kidney at any dose groups, in liver high and middle dose groups decrease. The content of T-SH and PB-SH increases in blood and brain of high dose groups. The content of NP-SH decreases in brain and kidney of high dose groups and in liver of middle and low dose groups. Comparing with control group, the difference is notable （P 〈0. 05 or P 〈0. 01 ）. Conclusion ： The liver and brain of mice have high sensitivity to free radical caused by Glyoxal , and they were damage easily by Lipid Peroxidation; The activity of GSH-Px in blood , liver and kidney is