三氧化二砷对前列腺癌细胞PC-3凋亡抑制蛋白XIAP表达的影响

目的]探讨三氧化二砷(As2O3)对前列腺癌细胞株PC-3细胞增殖、细胞凋亡及X染色体连锁凋亡抑制蛋白(XIAP)表达的影响.方法]取对数生长期PC-3细胞,分别经0.5,1.0,2.0μmol/L As2O3处理,采用四甲基偶氮唑蓝比色法检测细胞生长抑制作用;流式细胞仪检测细胞凋亡率;蛋白质印迹法及实时荧光定量PCR检测XIAP蛋白表达及XIAP mRNA表达的变化.结果]0.5,1.0,2.0μmol/LAs2O3均可抑制PC-3细胞增殖,在处理24,48,72h后,细胞生长抑制率间差异具有统计学意义(P<0.01).检测0.5,1.0,2.0μmol/L As2O3处理48h的PC-3细胞株细胞凋亡率分别为11.47%±1.81%,38.47%±1.60%,58.93%±3.35%,相比较差异具有统计学意义(P<0.01).As2O3处理48h时0.5,1.0,2.0μmol/L As2O3组的XIAP mRNA表达水平分别为0.67±0.12,0.25±0.15,0.03±0.01,各组间差异均具有统计学意义(P<0.05).结论]As2O3可抑制PC-3细胞增殖,诱导细胞凋亡,此作用可能与As2O3抑制PC-3细胞XIAP基因的表达有关联.

Effects of arsenic trioxide on expression of XIAP of human prostate cancer PC-3 cells

OBJECTIVE To study the effects of arsenic trioxide on the cell proliferation,apoptosis and expression of XIAP of prostate cancer PC-3 cells.METHODS The PC-3 cells of logarithmic growth phase were collected and were separately treated by 0.5,1.0,2.0 μmol/L of As2O3,and the effects of inhibiting proliferation of the PC-3 cells were analyzed by MTT assay,the apoptosis rate of PC-3 cells was measured by flow cytometry,the protein expression of XIAP and XIAP mRNA level of the cells were separately detected by the western blot and real time PCR.RESULTS 0.5,1.0,2.0 μmol/L of As2O3 were all inhibited the cell proliferation.After treated by As2O3 with different concentrations for 24,48,72 h,cell inhibition rates were significant difference(P<0.01).After treated by As2O3 with 0.5,1.0,2.0 μmol/L for 48 h,the apoptosis rate was 11.47%±1.81%,38.47%±1.60% and 58.93%±3.35%(P<0.01),respectively.After 48 h,the expression of XIAP mRNA from 0.5,1.0,2.0 μmol/L group was 0.67±0.12,0.25±0.15,and 0.03±0.01(P<0.05),respectively.CONCLUSION The arsenic trioxide can inhibit PC-3 cell proliferation and induce cell apoptosis,and its mechanism may be related to the inhibition of expression of XIAP of human prostate cancer PC-3 cells.