肝纤维化时血浆内皮素含量的动态观察

目的测定肝纤维化时血浆内皮素(ET)含量的动态改变.方法36只家兔随机分为正常组、病理组.于感染血吸虫尾蚴后第70,100,130天,每组随机选6只取血和肝分别作血浆ET含量和肝组织羟脯氨酸(HYP)含量测定.结果正常组各阶段间ET和HYP含量均无显著性差异(P＞0.05),病理组血浆ET含量和肝HYP与肝胶原纤维分布面积百分比均随着病程延长而增加.结论结果提示肝纤维化时血浆ET含量的增加可能参与了肝组织纤维化的进展.

Dynamic observation level of endothelin in plasma with liverfibrosis

Objective: To observe the level of the endothelin (ET) in plasma with liverfibrosis. Methods: 36 domestic rabbits were randomly and equally divided into normal group and pathologic group, on the 70 th, 100 th, 130 th day after being infected with cercariae, 6 rabbits taken randomly from each group were killed and prepared for plasma to determine the level of ET, prepared for liver tissue testing of the content of hydroxyproline (HYP) with ultravioletspectrophotometer, The percentage of collagenic fiber distribution in liver tissue was investigated under IBAS. Results: The results showed that there was no remarkable difference between plasmic ET level and HYP in the normal group (P>0.05), but the ET level and HYP of the pathologic group was gradually increased with the prolongation of disease process. The concentration of HYP and the percentage of collagenic fiber distribution were increased with the advance of the disease. Conclusions: The increasing of the ET level in plasma may participate in aggravation of the liver fibrosis.