化学发光酶免疫分析法及ELISA检测抗-HCV的结果比较

目的对比化学发光酶免疫分析法及酶联免疫吸附试验(ELISA)用于丙型肝炎抗体检测的效果。方法选取丙型肝炎患者140例,抽取血液标本后分别进行丙型肝炎病毒(HCV)相关抗体的化学发光酶免疫分析法及ELISA法检测。结果化学发光酶免疫分析法检出抗-HCV阳性的检出率为98.6%,高于ELISA法的检出率(94.3%),差异有统计学意义(P0.05)。化学发光酶免疫分析法对于抗AMA-M2抗体、抗3E抗体、抗SP100抗体、抗PML抗体、抗GP210抗体的检测阳性率分别为80.0%、73.6%、37.9%、55.7%和47.9%,而ELISA法检测结果分别为12.9%、12.9%、13.6%、10.7%和6.4%,差异均有统计学意义(P0.05)。结论相对于ELISA法,化学发光酶免疫分析法在丙型肝炎中的应用有较高的检出阳性率,对于相关抗体检测有较高的敏感性,值得推广应用。

Comparison analysis of effects of chemiluminescent enzyme immunoassay and ELISA for detection of hepatitis C antibod-ies

Objective To compare the effects of chemiluminescent enzyme immunoassay and ELISA method for the detection of hepatitis C antibodies.Methods 140 inpatients with hepatitis C in our hospital were selected and the blood samples were collected for detecting hepatitis C antibodies by adopting the chemiluminescence immunoassay and ELISA method.Results The positive detection rate of anti-hepatitis C virus(HCV)antibody detected by the chemiluminescent enzyme immunoassay was 98.6%,which was higher than 94.3% detected by the ELISA method, the difference was statistically significant (P <0.05 ).The positive rates of chemiluminescent enzyme immunoassay for detecting anti-AMA-M2 antibody,anti-3E antibody,anti-SP100 antibody,anti-PML antibody and anti-GP210 anti-body were 80.0%,73.6%,37.9%,55.7% and 47.9% respectively,while which detected by the ELISA method were 12.9%,12.9%,13.6%,10.7% and 6.4% respectively,the differences were statistically significant (P <0.05).Con-clusion Compared with the ELISA method,the chemiluminescent enzyme immunoassay has very good positive detec-tion rate in hepatitis C,has better sensitivity for the relevant antibody detection,at the same time has higher detection sensitivity and is worthy of being promoted and applied.