抗腺癌肿瘤疫苗对小鼠Lewis肺癌的主动特异性免疫治疗

目的：应用本室制备的抗腺癌肿瘤疫苗对小鼠高转移性ｌｅｗｉｓ肺癌进行防治研究，观察其抑癌、抗转移的效果对免疫功能的影响。方法：用肿瘤疫苗免疫Ｃ５７小鼠共４次，第三次免疫的同时小鼠右腋下接种ｌｅｗｉｓ肺癌。在既定日期检测移植瘤重及肺转移率、转移灶数。结果：瘤重，三种剂量疫苗组分别与对照生理盐水组，环磷酰胺组和ｒＩＬ－２组比较差异显著；肿瘤肺转移率及肺转移灶数疫苗组与各对照组比较差异显著。结论：该肿瘤疫苗有一定的抑癌和抗转移作用。

Active specific immunotherapy of a murine metastatic Lewis tumor using a cancer vaccine against adenocarcinoma

Objective:To observe the inhibiting roles of tumor growth and metastasis and the efficacy of immune response, the metastatic Lewis lung tumor bearing mice were inoculated a cancer vaccine against adenocarcinoma developed in this department.Methods:Three dosage vaccine groups were administered sc., totally 4 times. When the third vaccine immunization while the mice were received injections of metastatic Lewis lung tumor tissue suspension at subaxillary sc.. Then mice were sacrificed on limited day and examined their tumor weights, numbers of metastatic rates of lung. Results:All of mice represented tumors but the median weights of tumor, the vaccine groups were more light than the control groups (normal saline group, cyclophosphamide group and rIL 2 group, P <0 05~0 01);the metastatic rates of lung, the vaccine groups were lower than the control groups ( P <0 05~0 01) and the numbers of metastatic focus of lung, vaccine groups compared with control groups, displayed a significant difference ( P <0 001). Conclusion:This study indicates that our prepared tumor vaccine can be recognized by the mice immune system and may play the roles to inhibit tumor growth and metastasis.