新的白血病相关基因LRP16的克隆

目的 发现新的白血病相关基因及探讨白血病的发生机制。方法 应用分子克隆技术ＲＬＧＳ及ＲＡＣＥ技术自急性髓细胞白血病骨髓标本中克隆新的白血病相关基因。结果 克隆到一个长度为２９４８ｂｐ的ＤＮＡ片段，经国际基因库同源检索证明为一未知基因的一部分，并定位于第１１号染色体长臂１１区，进一步克隆到这一基因的全长ｃＤＮＡ，其长度为９８０ｂｐ，并被国际基因库以新的人类基因ＬＲＰ１６收录入库。结论 ＲＬＧＳ及ＲＡ

Cloning of leukemia associated gene LRP16 in acute myeloid leukemia

Objective:The purpose of this paper is to clone novel leukemia associated gene in acute myeloid leukemia (AML).Methods:The Restriction landmark genomic scanning (RLGS),Southern blot, Northern blot and rapid amplification of cDNA end (RACE) technique were performed on genomic DNA or RNA from AML and normal bone marrow (BM) samples.Results:An abnormal 2948bp fragment due to a distinct DNA methylation on NotI site in relapse sample from AML was cloned. The Sequence represents part of a new gene LRP16 which is located on chromosome 11q11. The full length cDNA sequence 980bp was cloned by RACE technique. Conclusion:The results indicate that RLGS and RACE technique are effective methods to find leukemia associated gene in AML. The finding of LRP16 gene is important to investigate mechanism of AML.