过氧化物酶体增殖物激活受体γC161→T基因多态性与高脂血症的膳食易感性研究

目的探讨过氧化物酶体增殖物激活受体γ(PPARγ)基因C161→T多态性在人群中的分布及其与血脂和膳食因素的关系。方法从血凝块中提取DNA,用聚合酶链反应和限制性片段长度多态性方法(PCR-RFLP)检测上海800名成年人的PPARγ基因C161→T位点多态性;对研究对象进行体格检查、膳食调查及血脂谱项目测定。结果(1)CC、CT和TT三种基因型的频率分别为35.1%、47.0%和17.9%,符合Hardy-Weinberg定律。高脂血症组人群和血脂正常组人群的基因型CC、CT和TT及等位基因C和T构成比差异无显著性,三种基因型及等位基因C和T在性别上无显著性差异。(2)调整性别、年龄和体质指数后,三种基因型的甘油三酯(TG)水平差异有显著性意义,CC型的TG最高。(3)调整性别和年龄后,三种基因型的蛋白质摄入量差异有显著性。(4)高血脂症组人群不同基因型人群中,总能量、蛋白质和碳水化合物与血清TG水平的负相关性有显著性意义。结论PPARγ基因C161→T位点多态性与TG水平有关,CC型的TG水平最高,和蛋白质摄入量有关联;该位点多态性可能会影响血清甘油三酯对膳食干预的易感性。

ASSOCIATION OF PEROXISOME PROLIFERATOR-ACTIVATED-RECEPTORS-GAMMA C161→T GENE POLYMORPHISM WITH HYPERLIPIDEMIA AND DIETARY PREDISPOSITION

Objective To study the genotype frequencies of peroxisome proliferator-activated- receptors- gamma (PPARγ) C161→T gene and its possible association with serum lipid levels and dietary intakes. Methods PCR-PFLP method was used to detect the polymorphism of PPARγC161→T gene of 800 adults in Shanghai. Their physical examinations,dietary survey and the levels of serum lipid profile , including TG、TC ,HDL and LDL were analyzed. Results (1) The genotype frequencies of PPARγC161→T CC,CT and TT were 35.1 %, 47.0 % and 17.9 % respectively , which were in agreement with Hardy- Weinberg equilibrium. There was no significant difference in distribution of genotypes or allele between the hyperlipidemia group and the control group and the result was same between male and female subjects. (2)After adjusting age,gender and body mass index, the levels of TG were significantly different among three genotypes groups,the highest in CC genotype. (3) After adjusting age and gender, the intakes of protein were significantly different among three genotype groups. (4) There was significant difference in the negative correlation between energy,protein and carbohydrate and serum TG levels in hyperlipidemia group.Conclusion PPARγ C161→T gene polymorphism influenced serum TG level, while associated with protein intakes. It might contribute to the heterogeneity in TG response to dietiary intervention.