| SARS冠状病毒Orf3和Orf4的克隆和分析 |
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| 引用本文： | 张洪勤,吴淑珍,毕云天,包其郁.SARS冠状病毒Orf3和Orf4的克隆和分析[J].温州医学院学报,2005,35(1):19-21. |
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| 作者姓名： | 张洪勤 吴淑珍 毕云天 包其郁 |
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| 作者单位： | 温州医学院,分子生物学研究中心,浙江,温州,325027 |
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| 基金项目： | 浙江省温州市科技局资助项目 |
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| 摘 要： | 目的:克隆SARS冠状病毒的Orf3和Orf4两个开放性读框,并对其进行DNA序列和蛋白质结构分析.方法:通过RT-PCR,从SARS冠状病毒基因组中扩增得到特异性片段,利用TA克隆将PCR产物克隆入PUCm-T载体并进行测序、同源性比较和蛋白质结构分析.结果:RT-PCR扩增出的特异产物与预期长度相符,序列分析表明,其核苷酸序列与已发表的SARS病毒的Orf3和Orf4同源性在99%以上.结论:成功克隆SARS冠状病毒Orf3和Orf4两个开放性读框,为表达及功能研究奠定了基础.
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| 关 键 词： | SARS冠状病毒 基因克隆 |
| 文章编号： | 1000-2138(2005)01-0019-03 |
| 修稿时间： | 2004年3月12日 |
Cloning and sequencing Orf3 and Orf4 of the SARS coronavirus |
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| ZHANG Hong-qin,WU Shu-zhen,BI Yun-tian,et al..Cloning and sequencing Orf3 and Orf4 of the SARS coronavirus[J].Journal of Wenzhou Medical College,2005,35(1):19-21. |
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| Authors: | ZHANG Hong-qin WU Shu-zhen BI Yun-tian |
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| Institution: | ZHANG Hong-qin,WU Shu-zhen,BI Yun-tian,et al. Molecular Biology Research Centre of Wenzhou Medical College,Wenzhou 325027 |
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| Abstract: | Objective:To clone and analyze Orf3 and Orf4 of the SARS coronavirus.Methods:Orf3 and Orf4 were obtained by RT-PCR from the SARS coronavirus genome .RT-PCR products were ligated into pUCm-T vector and sequenced.Results:Tow cloned segments were identical in length and these sequences were over 99% identical to Orf3 and Orf4 of all SARS genomes in Genebank data.Conclusion:Orf3 and Orf4 of the SARS coronavirus are successfully cloned using RT-PCR,which could be used for expression and functional research. |
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| Keywords: | Orf3 Orf4 |
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