Biochemical and histological studies on various bone cell preparations |
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Authors: | P J Nijweide A van der Plas J P Scherft |
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Institution: | (1) Department of Cell Biology and Histology, University of Leiden, Rijnsburgerweg 10, Leiden, The Netherlands |
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Abstract: | Summary Four different cell populations—designated PF, OB, OC, and PC—were isolated from calvaria of 18-day-old chick embryos for
analysis of the effects of hormones on bone tissue. The cell populations were studied with histological and biochemical methods.
Apart from the well-known cell types present in calvaria, a new cell type was found in the noncalcified organic matrix between
the osteoblastic layer and the calcified matrix. These cells were provisionally called osteocytic osteoblasts. They represent
the “transition state” between osteoblasts and osteocytes.
On the basis of histological studies with light microscopy (LM), transmission electron microscopy (TEM) and scanning electron
microscopy (SEM), the PF population was considered to originate primarily from the periosteal fibroblasts, the OB population
from the osteoblasts and osteocytic osteoblasts. The population of cells still present in calvaria after removal of periosteal
fibroblasts and osteoblasts was called the OC population. This cell population was very much enriched with osteocytes. The
fourth isolated population (PC) was a mixed population of fibroblasts, osteoblasts, and preosteoblasts. On exposure to parathyroid
hormone (PTH), all four cell populations showed increased lactate production, but only the OB and OC populations displayed
increased cAMP production. Prostaglandin E1 (PGE1) stimulated cAMP production in both OB and PF cells.
From the results of this study it was concluded that PTH receptors are present on all of the cell types studied, but that
occupancy of the receptor induces adenylate cyclase stimulation only in osteocytes and fully differentiated osteoblasts. |
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Keywords: | Bone cells Electron microscopy PTH PGE1 |
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