糖尿病肾病患者血清同型半胱氨酸与氧化应激的关系

目的：探讨血清高同型半胱氨酸（Hcy）导致的氧化应激与糖尿病肾病的关系。方法：随机抽取50例健康者为对照组，将150例2型糖尿病患者按照尿白蛋白水平分为正常蛋白尿组、微量白蛋白尿组及临床蛋白尿组，各50例。用ELISA法检测血清Hcy，分光光度计法测定血清中超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、丙二醛（MDA）的含量。结果：糖尿病患者血清Hcy、MDA含量均高于对照组(P<0.01)；微量白蛋白尿组及临床白蛋白尿组高于正常白蛋白尿组 (P<0.01)；血清SOD、GSH含量所有糖尿病患者低于正常对照组(P<0.01)；微量白蛋白尿组及临床白蛋白尿组低于正常白蛋白尿组(P<0.01)；血清同型半胱氨酸水平与血清中MDA水平呈正相关（r =0.64, P<0.01），与SOD、GSH含量均呈负相关（r分别为 -0.57,-0.62, 均P <0.01。）。结论：血清同型半胱氨酸导致氧化应激损伤参与糖尿病肾病的发生发展过程。

The Relationship between the Serum Homocysteine and Oxidative Stress in Patients with Diabetic Nephropathy

Objective to investigate the relationship between the content of serum maleic dialdehyde （MDA）, superoxide disdmutase （SOD）and reduced glutathione （GSH） with complication of type 2 diabetic mellitus. the influence and mechanism of oxidaive stress on diabetic nephropathy，so as to ofter theoretic evidences for clinical therapy. Methods：50 cases of the control group of healthy patients；150 cases with type 2 diabetes mellitus is divided into three groups of patients with normal albuminuria group, microalbuminuria and clinical proteinuria group, each group contens 50 cases. ELISA assay with serum homocysteine, spectrophotometer serum MDA, SOD, GSH content. Results：All patients with diabetes serum homocysteine, MDA levels were significantly higher than the normal control group (P <0.01); micro-albuminuria albuminuria group and clinical group were higher than normal albuminuria group and normal control group (P <0.01); clinical albuminuria group was higher than the microalbuminuria group (P <0.01); Serum SOD, GSH content of all the diabetic patients were lower than normal control group (P <0.01); micro-albuminuria albuminuria group and clinical group lower than those without nephropathy (P <0.01); serum homocysteine level and the level of serum MDA was positively correlated with serum homocysteine levels and SOD, GSH content showed a negative correlation（ P <0.01）. Conclusion：Serum homocysteine damage through oxidative stress involved in the occurrence of diabetic nephropathy development process.