Role of Oxidative Stress in a Rat Model of Radiation-Induced Erectile Dysfunction |
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| Authors: | Masaki Kimura Zahid N. Rabbani Andrew R. Zodda Hui Yan Isabel L. Jackson Thomas J. Polascik Craig F. Donatucci Judd W. Moul Zeljko Vujaskovic Bridget F. Koontz |
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| Affiliation: | 2. Department of Radiation Oncology and Duke Prostate Center, Duke University Medical Center, Durham, NC, USA;2. Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea;2. Center for Demography and Health Research, Nicaraguan National Autonomous University, León, Nicaragua;2. Nuclear Med. and Mol. Imaging, Univ. Med. Ctr. Groningen, Groningen, the Netherlands;3. Obstetrics and Gynecology, Univ. Med. Ctr. Groningen, Groningen, the Netherlands;4. Dept. of Sexology and Psychosomatic Obstetrics and Gynecology, Academic Med. Ctr., Amsterdam, the Netherlands;5. Boehringer-Ingelheim Pharma., Inc., Alkmaar, the Netherlands;2. Department of Pediatrics, Clinical Genetics Unit, University of Padova, Padova, Italy;3. Department of Environmental Medicine and Public Health, University of Padova, Padova, Italy;2. Department of Health Science, University of Arkansas, Fayetteville, AR, USA;3. Institute for Urologic Excellence, Indigo, CA, USA;4. Department of Biostatistics, University of Arkansas for Medical Sciences, Little Rock, AR, USA;5. University of Michigan Medical School, Ann Arbor, MI, USA |
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| Abstract: | IntroductionChronic oxidative stress is one of the major factors playing an important role in radiation-induced normal tissue injury. However, the role of oxidative stress in radiation-induced erectile dysfunction (ED) has not been fully investigated.AimsTo investigate role of oxidative stress after prostate-confined irradiation in a rat model of radiation-induced ED.MethodsFifty-four young adult male rats (10–12 weeks of age) were divided into age-matched sham radiotherapy (RT) and RT groups. Irradiated animals received prostate-confined radiation in a single 20 Gy fraction.Main Outcome MeasuresIntracavernous pressure (ICP) measurements with cavernous nerve electrical stimulation were conducted at 2, 4, and 9 weeks following RT. The protein expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase subunits (Nox4 and gp91phox), markers of oxidative DNA damage (8-hydroxy-2′-deoxyguanosine [8-OHdG]), lipid peroxidation (4-hydroxynonenal [4HNE]), and inflammatory response including inducible nitric oxide synthase, macrophage activation (ED-1), and nitrotyrosine, and endogenous antioxidant defense by nuclear factor erythroid 2-related factor (Nrf2) were evaluated in irradiated prostate tissue and corpora cavernosa (CC). In addition, we investigated the relationships between results of ICP/mean arterial pressure (MAP) ratios and expression level of oxidative stress markers.ResultsIn the RT group, hemodynamic functional studies demonstrated a significant time-dependent decrease in ICP. Increased expression of Nox4, gp91phox, 8-OHdG, and 4HNE were observed in the prostate and CC after RT. Similarly, expressions of inflammatory markers were significantly increased. There was a trend for increased Nrf2 after 4 weeks. ICP/MAP ratio negatively correlated with higher expression level of oxidative markers.ConclusionsNADPH oxidase activation and chronic oxidative stress were observed in irradiated prostate tissue and CC, which correlated with lower ICP/MAP ratio. Persistent inflammatory responses were also found in both tissues after RT. These findings suggest that oxidative stress plays a crucial role in the development of radiation-induced ED. Kimura M, Rabbani ZN, Zodda AR, Yan H, Jackson IL, Polascik TJ, Donatucci CF, Moul JW, Vujaskovic Z, and Koontz BF. Role of oxidative stress in a rat model of radiation-induced erectile dysfunction. J Sex Med 2012;9:1535–1549. |
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