Blocking effect of sperm immobilizing antibodies on sperm penetration of human zonae pellucidae |
| |
| Authors: | Shinichi Tsukui Yoichi Noda Aisaku Fukuda Hisashi Matsumoto Kenichi Tatsumi Takahide Mori |
| |
| Institution: | (1) Department of Obstetrics and Gynecology, Osaka Red Cross Hospital, 5-53 Fudegasaki-cho, Tennoji-ku, 543 Osaka, Japan;(2) Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Kyoto, Japan |
| |
| Abstract: | To investigate the mechanism of the blocking effect of sperm immobilizing antibodies on human fertilization, an in vitro zona penetration test was carried out using media containing the IgG fraction extracted from sperm immobilizing antibody-negative or-positive serum. The sperm penetration rate of the test was 100% (6/6) when spermatozoa were treated with the IgG fraction derived from sperm immobilizing antibody-negative serum, whereas it was only 17% (1/6) when spermatozoa were treated with the IgG fraction derived from sperm immobilizing antibody-positive serum. Electron microscopic observation of the sperm immobilizing antibody-negative and-positive serum-treated spermatozoa showed that the number of acrosome-reacted spermatozoa was significantly greater in the sperm immobilizing antibody-negative serum than in the antibody-positive serum. Therefore, it appears that one of the blocking mechanisms of the spermatozoal penetration of the zona pellucida by sperm immobilizing antibodies may be due to inhibition of the acrosome reaction in the spermatozoa. |
| |
| Keywords: | sperm immobilizing antibody zona pellucida acrosome reaction highly concentrated salt solution |
| 本文献已被 SpringerLink 等数据库收录！ |
|
