PDK1在胰腺癌中的表达及其临床意义

目的:探讨PDK1在胰腺癌中的表达及其与临床病理特征的关系.方法:对44例胰腺癌及其癌旁正常组织标本,分别应用RT-PCR方法和Western blot方法检测其PDK1的mRNA和蛋白表达,并和临床病理指标进行统计学分析.结果:经RT-PCR检测胰腺癌组织中PDK1表达水平明显高于癌旁正常组织(0.352006 vs 0.074887,P<0.01);Western blot检测发现72.727%(32/44)PDK1蛋白的表达癌组织中高于癌旁组织,有统计学差异(P<0.01).PDK1在胰腺癌组织中的表达与患者性别、年龄、肿瘤大小和分化程度均无相关性(P>0.05),而与T分期、淋巴结转移和TNM分期密切相关(P<0.05).结论:PDK1在胰腺癌中表达上调,可能与胰腺癌发生、发展及转移有关.

Clinical signif icance of expression of PDK1 in human pancreatic cancer

AIM:To investigate the expression of PDK1 in human pancreatic cancer and to analyze its correlation with clinicopathological features of this pancreatic malignancy.METHODS:The mRNA and protein expression of PDK1 was detected in 44 pancreatic cancer tissue samples and matched tumor-adjacent normal tissue samples by real-time quantitative PCR and Western blot,respectively.Then,the relationship between expression of PDK1 and clinicopathological features of pancreatic cancer was analyzedpancreatic tissue(0.352006 vs 0.074887,P < 0.05).Western blot analysis showed a higher positive rate of PDK1 protein expression in cancer tissue than in adjacent tissue.Expression of PDK1 had no signif icant correlation with gender,age,tumor size or differentiate type(all P > 0.05),but was related to T stage,lymph nodes metastasis and TNM stage(all P < 0.05).CONCLUSION:Up-regulation of PDK1 expression in pancreatic cancer suggests that PDK1 may be involved in the carcinogenesis,development and metastasis of pancreatic cancer.