Assessment of immunological mechanism in infertility of the rat after experimental testicular torsion |
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Authors: | E. Merimsky R. Orni-Wasserlauf I. Yust |
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Affiliation: | (1) Department of Urology, Municipal Governmental Medical Center, Tel Aviv University Medical School, Ichilov Hospital, Tel Aviv-Jaffo, Israel;(2) Department of Medicine A, Municipal Governmental Medical Center, Tel Aviv University Medical School, Ichilov Hospital, Tel Aviv-Jaffo, Israel;(3) The Clinical Immunology Unit, Municipal Governmental Medical Center, Tel Aviv University Medical School, Ichilov Hospital, Tel Aviv-Jaffo, Israel |
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Abstract: | Summary A necrotic torted testis left in the scrotum affects the contralateral testis, resulting in depressed fertility. It was suggested that testicular torsion provoked a systemic response of an immunologic nature. In order to prove this theory, 56 rats were operated. Ten had a sham operation, in 17 a testis was torted and excised after 24 h, and in 29 a testis was torted and excised after 24 h, and in 29 a testis was torted but left in situ till the sacrifice. Antisperm-antibodies were not found in any phase of the experiment in the sham group. Using indirect immunofluorescence, antisperm-antibodies were found in the sera of 8/17 rats of the orchiectomy group and in 24/29 of the detorted group. In the direct immunofluorescence study, all the antibodies were localized in the tail of the spermatozoid. The antisperm-antibodies dissappeared after 3 to 6 months. |
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Keywords: | Testicular torsion Immunologicol infertility Autoimmune orchitis Spermatogenesis Antisperm-antibodies Immunofluorescence |
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