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| RP-HPLC法测定参茸固本片中补骨脂素和异补骨脂素含量 | |
| 引用本文： | 李亚,张成川,宋剑锋.RP-HPLC法测定参茸固本片中补骨脂素和异补骨脂素含量[J].海峡药学,2011,23(4):57-59. |
| 作者姓名： | 李亚 张成川 宋剑锋 |
| 作者单位： | 1. 浙江临安市人民医院,杭州,311300 2. 浙江乐清市中医院,温州,325000 3. 浙江衢州市药品检验所,衢州,324002 |
| 摘 要： | 目的建立一种可测定参茸固本片中补骨脂素和异补骨脂素的RP-HPLC方法。方法采用Shim-pack VP-ODS（250mm×4.6mm5μm）为色谱柱;流动相为甲醇-水（45∶55）;检测波长为246nm;流速为1.0mL.min-1;柱温为40℃;峰面积外标法定量;结果本法补骨脂素在9.10×10-4μg~0.2742μg范围内呈良好的线性关系,（r=0.99999）,平均回收率为99.0%,RSD 1.57%;异补骨脂素在9.90×10-4μg~0.2970μg范围内呈良好的线性关系,（r=0.99999）,平均回收率为100.7%,RSD 1.76%;结论本方法简便、快速、准确可靠,可作为该制剂的质控方法。 |
| 关 键 词： | 参茸固本片 补骨脂素 异补骨脂素 高效液相色谱法 含量测定 |
Determination of psoralen and isopsoralen in Shenrong Guben Tablets by RP-HPLC |
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| LI ya,ZHANG Cheng-chuan,SONG Jian-feng.Determination of psoralen and isopsoralen in Shenrong Guben Tablets by RP-HPLC[J].Strait Pharmaceutical Journal,2011,23(4):57-59. | |
| Authors: | LI ya ZHANG Cheng-chuan SONG Jian-feng |
| Institution: | 1.The people’s hospital of Lin’an city,hangzhou 311300,China;2.Yueqing Hospital of Traditional Chinese Medicine,Wenzhou 325200,China;3.Quzhou Institute for Drug Control;Quzhou 324002,China) |
| Abstract: | OBJECTIVE To establish a RP-HPLC method for determining the content of Psoralen and isopsoralen in Shenrong Guben Tablets.METHODS A Shim-pack C-(18)column(250mm×4.6mm 5μm)was used.The mobile phase was methanol-water(45∶55).The detection wavelength was 246 nm.RESULTS The linear range of paoralen was 9.10×10-4μg～0.2742μg(r=0.999 99).The average recovery(n=6)was 99.0%.RSD was 1.57%.The linear range of isopaoralen was 9.90×10-4μg～0.2970μg(r=0.999 99).The average recovery(n=6)was 100.7%.RSD was 1.76%.CONCLUSION The method is simple,reliable,accurate and can be applied to the quality control of the preparetion. |
| Keywords: | Shenrong Guben Tablets Psoralen Isopsoralen RP-HPLC Content determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |