| 阿柔比星与人血白蛋白相互作用的特点 |
| |
| 引用本文： | 徐晓卫,梁晶,林观样.阿柔比星与人血白蛋白相互作用的特点[J].海峡药学,2011,23(4):205-207. |
| |
| 作者姓名： | 徐晓卫 梁晶 林观样 |
| |
| 作者单位： | 浙江温州医学院附属第一医院药剂科,温州,325000 |
| |
| 基金项目： | 基金资助:温州市科技局基金资助项目 |
| |
| 摘 要： | 目的研究阿柔比星与人血清白蛋白的相互作用特点。方法用荧光猝灭光谱及同步荧光光谱技术测定在不同温度下阿柔比星对人血清白蛋白荧光的猝灭规律。结果阿柔比星对人血清白蛋白荧光呈规律性猝灭,17℃时的n为1.11,K为6.21×104,37℃时的n为1.12,K为6.41×104。阿柔比星使白蛋白的同步光谱中最大发射峰红移。结论阿柔比星与人血清白蛋白只有一个结合位点,表现为疏水作用,两者结合使白蛋白的极性略有增加。
|
| 关 键 词： | 荧光光谱 阿柔比星 人血清白蛋白 相互作用 |
Interaction between adriamycin and human serum albumin |
| |
| XU Xiao-wei,LIANG Jing,LIN Guan-yang.Interaction between adriamycin and human serum albumin[J].Strait Pharmaceutical Journal,2011,23(4):205-207. |
| |
| Authors: | XU Xiao-wei LIANG Jing LIN Guan-yang |
| |
| Institution: | (Department of pharmacy,The first affiliated hospital of wenzhou medical college,wenzhou 325000,China) |
| |
| Abstract: | OBJECTIVE To study the interaction between adriamycin and human serum albumin.METHODS Fluorescence quenching spectroscopy and synchronous fluorescence spectrometry were used.RESULTS It showed that the fluorescence intensity of HSA was quenched intensively by adriamycin.The combining parameters between strychnine and HSA are:17℃:n=1.11,K=6.21×104;37℃:n=1.12,K=6.41×104,respectively.The maximum peek is changed to long wave on its synchronous fluorescence(Δλ=60nm).CONCLUSION The interaction between strychnine and BSA was driven by hydrophobic force.The combining site is one,and the polarity of HSA maybe improve when it combine adriamycin. |
| |
| Keywords: | Fluorescence spectroscopy Adriamycin Human serum albumin Interaction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
