| HPLC-RID法测定氨基葡萄糖盐酸盐的含量 |
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| 引用本文： | 李俊. HPLC-RID法测定氨基葡萄糖盐酸盐的含量[J]. 海峡药学, 2011, 23(2): 42-44 |
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| 作者姓名： | 李俊 |
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| 作者单位： | 浙江台州市食品药品检验所,台州,318000 |
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| 摘 要： | 目的 建立高效液相色谱-示差折光检测法测定氨基葡萄糖盐酸盐的含量.方法 采用高效液相色谱法,色谱柱为Bondapak C18柱(3.9×300mm,10μm),流动相为0.005mol·L-1庚烷磺酸钠溶液(pH4.0)-乙腈(97:3),流速:2.0mL·min-1,柱温25℃,示差折光检测器池温度40℃.结果 本法...
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| 关 键 词： | HPLC 示差折光检测器 氨基葡萄糖盐酸盐 含量测定 |
Determination of glucosamine hydrochloride by RID-HPLC |
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| LI Jun. Determination of glucosamine hydrochloride by RID-HPLC[J]. Strait Pharmaceutical Journal, 2011, 23(2): 42-44 |
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| Authors: | LI Jun |
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| Affiliation: | LI Jun(Taizhou institute for food and drug control,Taizhou 318000,China) |
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| Abstract: | OBJECTIVE An RID-HPLC method was established to determine the content of glucosamine hydrochloride.METHODS Using a HPLC method with Bondapak C18 column(3.9×300mm,10μm),the mobile phase was 0.005mol·L-1 1-heptanesulfonic acid sodium salt solution(pH 4.0) acetonitrile(97∶3),the flow rate was 2.0mL·min-1,acolumn temperature was 25℃ and the cell temperature of Refractive Index Detector was 40℃.RESULTS Precision and stability of the method were fine,The standard curve was linear in the range of 0.998～7.497mg·mL-1(r=0.9999),and the average recovery was 99.4% with RSD of 1.2%.CONCLUSION The method was simple,accurate and reproducible,and can be used for the method of determination. |
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| Keywords: | HPLC |
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