HPLC测定氟康唑莫西沙星眼用即型凝胶的含量

目的建立高效液相色谱法测定氟康唑莫西沙星眼用即型凝胶含量,同时测定制剂中氟康唑、莫西沙星和羟苯乙酯的含量。方法采用ODS-C18色谱柱,以三乙胺缓冲液-甲醇为流动相,流速1.0 mL.min 1,检测波长260 nm。结果氟康唑、莫西沙星、羟苯乙酯的线性范围分别为25～250 g.mL 1(r=0.999 9,n=6),25～250 g.mL 1(r=0.999 9,n=6),2.5～25 g.mL 1(r=0.999 7,n=6),平均回收率分别为99.6%,99.4%,99.3%。RSD分别为1.23%,1.20%,1.17%。结论本方法操作简便,灵敏度高,重复性好,可用于该制剂的含量测定。

Determination of Fluconazole and Moxifloxacin in Situ Forming of Eye Gel by HPLC

OBJECTIVE To establish an HPLC method for measuring the content of fluconazole, moxifloxacin and ethylparaben in situ forming of eye gel. METHODS ODS-C18 column was used, triethylamine buffer-methanol as mobile phase, the flow rate was 1.0 mL·min^-1, detection wavelength was 260 nm. RESULTS The linear range of fluconazole, moxifloxacin and ethylparaben were 25-250 μg·mL-l（r=0.999 9, n=6）, 25-250 μg·mL-l（r=0.999 9, n=6）, 2.5-25 p.g.mL-1 （r=0.999 7, n=6）, the average recovery rates were 99.6%, 99.4%, 99.3%. The RSD were 1.23%, 1.20%, 1.17%. CONCLUSION The method is simple, accurate, sensitive and the reproductivity is good. It can be used for the determination of this preparation.