| 碱性磷酸酶-抗碱性磷酸酶铅沉淀技术及其优化 |
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| 引用本文： | 刘能保,R.Bock,K.Chakraverty-Werner,G.Herth,E.stermann,U.Tx. 碱性磷酸酶-抗碱性磷酸酶铅沉淀技术及其优化[J]. 华中科技大学学报(医学版), 1992, 0(1) |
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| 作者姓名： | 刘能保 R.Bock K.Chakraverty-Werner G.Herth E.stermann U.Tx |
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| 作者单位： | 同济医科大学基础医学部组织胚胎学教研室(刘能保)，德国萨尔州大学理论医学部解剖学研究所(R.Bock，K.Chakraverty-Werner，G.Herth，E.stermann)，德国萨尔州大学理论医学部解剖学研究所(U.Tx) |
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| 摘 要： | 本文介绍了用以显示碱性磷酸酶活性的铅沉淀法的碱性磷酸酶-抗碱性磷酸酶(APA-AP)免疫组织化学技术,以及使用计算机图像处理进行显微光密度测量确定的该技术理想反应条件.其条件是:孵育液硝酸铅浓度为3mmol/L,底物浓度1.96～31.36mmol/L,pH值7～9;APAAP浓度1:50;一级抗体、第一和第二桥抗体的浓度分别为1:1 000、1:100和1:200。讨论了APAAP-铅沉淀技术的某些优点。
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| 关 键 词： | 碱性磷酸酶-抗碱性磷酸酶复合物 计算机 图像处理系统 显微光密度测量 免疫组织化学 |
An Alkaline Phosphatase-anti-Alkaline Phosphatase-Lead Capture Technique and its Optimization |
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| Liu Nengbao Department o/ Histology and Embryology,Faculty of Basic Medical Sciences,Tongji Medical University,WuhanR.Bock,K.Chakraverty-Werner,G.Herth,E.Ostermann,U.Tox Institute of Anatomy,Faculty of Theoretical Medicine,University of Saar State,Homburg,FRG. An Alkaline Phosphatase-anti-Alkaline Phosphatase-Lead Capture Technique and its Optimization[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1992, 0(1) |
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| Authors: | Liu Nengbao Department o/ Histology Embryology Faculty of Basic Medical Sciences Tongji Medical University WuhanR.Bock K.Chakraverty-Werner G.Herth E.Ostermann U.Tox Institute of Anatomy Faculty of Theoretical Medicine University of Saar State Homburg FRG |
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| Abstract: | A lead capture method was introduced,that demonstrates the activity of alkaline phophatase immunohistochemically for alkaline phosphatase-anti-alka-line phosphatase (APAAP) technique. By means of microdensitometry and computer aided image processing, the optimal reaction conditions of the method have been established, that is, the concentration of lead nitrate in the incubative medium is 3 mmol/L and that of the substrate is 1.96-31.36 mmol/L, the range of pH is between 7 and 9, the concentration of APAAP is 1:50 and that of primary antibody, the first and the second bridge antibodies is 1:1000, 1: 100 and 1:200 respectively. Advantages of the APAAP-lead technique were also discussed. |
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| Keywords: | alkaline phosphatase-anti-alkaline phosphatase complex computer image processing system microdensitometry immunohistochemistry |
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