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蒙药森登HPCE法的指纹图谱研究
引用本文:徐都冷,红颜,巴根那,拉喜那木吉拉,王伟.蒙药森登HPCE法的指纹图谱研究[J].中国民族医药杂志,2011,17(1):31-33.
作者姓名:徐都冷  红颜  巴根那  拉喜那木吉拉  王伟
作者单位:1. 内蒙古民族大学蒙医药学院,内蒙古,通辽,028000
2. 辽宁省阜新市蒙医研究所,辽宁,阜新,123000
摘    要:目的:建立蒙药材森登的高效毛细管电泳(HPCE)指纹图谱分析方法为蒙药材森登质量评价提供依据。方法:采用石英毛细管柱(内径75μm有效长度50cm)HPCE工作条件:分离电压20kv,柱温25℃,紫外检测器(UV)检测波长为254nm,缓冲溶液为50mmol·L^硼砂-100mmloL·L^硼酸-乙腈(2:1:1)混合溶液(pH=8.6)。结果:对7种不同产地的森登进行了分析,初步建立了以6个共有峰为特征指纹信息的HPCE指纹图谱。结论:HPCE指纹图谱法可用于蒙药材森登的质量评价。

关 键 词:HPCE法  指纹图谱  蒙药材  高效毛细管电泳  石英毛细管柱  质量评价  谱分析方法  紫外检测器

Study on Mongolia medicinal material senden fingerprint by means of HPCE
XU Duleng,HONG Yan,BAGENA,LAXINAMJILA,WANG Wei.Study on Mongolia medicinal material senden fingerprint by means of HPCE[J].Journal of Medicine & Pharmacy of Chinese Minorities,2011,17(1):31-33.
Authors:XU Duleng  HONG Yan  BAGENA  LAXINAMJILA  WANG Wei
Institution:Mongolia Medicine College of Inner Mongolia National University, Tongliao 0928000, China 1 Mongolia medicine Institute of fuxin city, fuxin city 123000, Liaoning province
Abstract:Objective:To establish a Mongohan medicine senden by high perfomanee capillary electrophoresis(HPCE) mthod for fingerprint analysis of the quality evaluation of Mongolia herbs provide the basis senden. Methods: quartz capillary eolumn(75μm inner diameter effective length 50cm) HPCE working conditions: separation voltage of 20kv, and the column temperature was 25℃, UV detector(UV)detection at 254nm, buffer solution to 50mmol/L borax100mmol/L boric acid - acetonitrile(2 : 1 : 1 ) mixed solution ( pH = 8.6). Result: seven kinds of different origin were analyzed senden, initially established with a total of six peaks for HPCE fingerprint fingerprint information. Conclu-sion:HPCE Fingerprinting Mongolian medicine can be used to evaluate the quality senden.
Keywords:Mongolian medicine senden  high performance capillary electrophoresis  fingerprint
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