| 外周血单核细胞中丙型肝炎病毒RNA正负链检测的临床意义 |
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| 引用本文： | 吴海滨,李智伟,李颖.外周血单核细胞中丙型肝炎病毒RNA正负链检测的临床意义[J].世界华人消化杂志,1999,0(3):220-221. |
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| 作者姓名： | 吴海滨 李智伟 李颖 |
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| 作者单位： | 110003,辽宁省沈阳市路号,国医科大学附属二院传染科. |
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| 摘 要： | 目的通过对外周血单核细胞（ＰＢＭＣ）中ＨＣＶＲＮＡ正负链的检测，来探讨其与丙型肝炎慢性化及干扰素治疗的关系．方法慢性丙型肝炎患者４０例，其中干扰素治疗者１０例，分离血清及ＰＢＭＣ．异硫氰酸胍－酚－氯仿抽提法提取ＨＣＶＲＮＡ，应用逆转录－巢式ＰＣＲ技术检测ＨＣＶＲＮＡ正负链．结果血清正链ＨＣＶＲＮＡ阳性率为６７５％，但负链均为阴性，ＰＢＭＣ中正链ＨＣＶＲＮＡ阳性率为５７５％，负链的阳性率为３５０％．其中３例患者血清中正链ＨＣＶＲＮＡ为阴性，而ＰＢＭＣ中为阳性．１０例干扰素治疗者在治疗结束时血清正链ＨＣＶＲＮＡ６０％转阴，ＰＢＭＣ中负链ＨＣＶＲＮＡ８０％转阴，而正链仅３７５％转阴．结论ＨＣＶ能在ＰＢＭＣ中存在和复制，这可能是导致丙型肝炎易发生慢性化的原因之一．ＰＢＭＣ中ＨＣＶＲＮＡ正负链的检测对于临床判断干扰素的疗效及预后有重要意义
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| 关 键 词： | 单核细胞 丙型肝炎病毒 RNA.病毒 干扰素／治疗应用 |
Clinical significance of detection of positive and negative strands of HCV RNA in peripheral blood mononuclear cells |
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| WU Hai Bin,LI Zhi Wei and LI Ying The Second Affiliated Hospital,China Medical University,Shenyang ,Liaoning Province,China.Clinical significance of detection of positive and negative strands of HCV RNA in peripheral blood mononuclear cells[J].World Chinese Journal of Digestology,1999,0(3):220-221. |
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| Authors: | WU Hai Bin LI Zhi Wei and LI Ying The Second Affiliated Hospital China Medical University Shenyang Liaoning Province China |
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| Institution: | WU Hai Bin,LI Zhi Wei and LI Ying The Second Affiliated Hospital,China Medical University,Shenyang 110003,Liaoning Province,China |
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| Abstract: | |
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| Keywords: | monocytes hepatitis C virus RNA viral interferon/therapeutic effect |
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