特发性血小板减少性紫癜患者激素敏感性与糖皮质激素受体亚型的关系研究

目的探究特发性血小板减少性紫癜（ITP）患者对糖皮质激素敏感性与其外周血单个核细胞内激素受体亚型表达的关系。方法采用实时定量RT—PCR和免疫组化技术检测不同激素敏感性ITP患者（包括糖皮质激素抵抗和敏感病人）外周血单个核细胞内α和β亚型糖皮质激素受体（GRα和GRβ）mRNA和蛋白表达，并将检测结果与正常对照组进行比较。结果糖皮质激素抵抗组患者外周血单个核细胞GRβmRNA表达量和GRβ蛋白表达阳性的单个核细胞比率明显高于激素敏感组及正常对照组（P〈0．01），而GRα mRNA和蛋白表达水平，各组之间无显著差异。结论ITP患者糖皮质激素敏感性与其GRβ表达水平密切相关。

Study on the Relationship Between Sensitivity to Glucocorticoid and Glucocorticoid Receptor Isoform in Patients with Idiopathic Thrombocytopenia Purpura

Objective To investigate the relationship between sensitivity to glucocorticoid and glucocorticoid receptor （GR） isoforms in peripheral blood mononuclear cells （PBMC） isolated from patients with idiopathic thrombocytopenia purpura （ITP）. Methods Real Time quantitative RT- PCR and immunocytochemical assay were used to measure the expression of and isoforms of glucocorticoid receptors （GRα and GRβ） in PBMC isolated from glucocorticoid-sensitive and resistant ITP patients as well as normal control groups. The expression of GRα and GRβ at mRNA and protein level among these groups were further analyzed and statistically compared. Results glucocorticoid-resistant ITP patients was It was found that the mRNA level of GRβ in PBMC isolated from significantly higher than that from glucocorticoid-sensitive ITP patients as well as control groups （P 〈 0.01 ）. Furthermore, the ratio of GRβ-positive PBMC was dramatically higher in glucocorticoid-resistant ITP group in comparison with that in glucocorticoid-sensitive and control groups （P 〈 0.01 ）. However, the mRNA and protein level of GRα showed little difference among these groups. Conclusion The response of ITP patients to glucocorticoid treatment maybe closely associated with GRβ expression in their PBMC.