重复无创肢体缺血预适应对大鼠心肌缺血再灌注损伤的保护作用

目的研究重复多次无创肢体缺血预适应对大鼠心肌缺血再灌注损伤的保护作用。方法 SD大鼠24只随机分为对照组、单次无创肢体预适应(LPC)组、反复无创后肢缺血预适应(RLPC)组各8只,观察重复无创性肢体缺血预适应对大鼠心脏缺血再灌注损伤的作用。通过颈动脉插管法测定大鼠的平均动脉压(MAP),肢体Ⅱ导联记录心电图以分析心率、心律失常情况。实验结束后采血测定血清中的丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活性,留取心脏染色测定各组梗死面积。结果与对照组相比,LPC组和RLPC组可以减小梗死面积,减轻心律失常情况,血清MDA浓度降低,SOD,GSH-PX的活性增加。但LPC组和RLPC组差异无统计学意义。结论 RLPC对大鼠缺血再灌注损伤的保护作用与LPC相似。

Protective effects of repetition limb ischemic preconditioning on cardiac ischemia-reperfusion injury in rats

Objective To determine the protective effects of repetition limb ischemic preconditioning （RLPC） on cardiac ischemia-reperfusion injury. Methods Sprague-Dawley rats were randomly assigned to control group （group C）, limb ischemic preconditioning group （group LPC） and repetition limb ischemic preconditioning group （group RLPC）, respectively, thus allowing for determination of the protective effects of RLPC on cardiac ischemia-reperfusion injury. Mean arterial pressure （MAP） was measured via cervical catheterization, and incidence of cardiac arrhythmia was monitored based on lead II electrocardiogram and the heart rate. The levels of serum malondialdehyde （MDA） and superoxide dismutase （SOD） and activity of glutathione peroxidase （GSH-PX） were assayed at the end of study. Furthermore, the size of myocardial infarction was assessed by pathologic staining. Results Compared with control group, both LPC and RLPC resulted in reduced size of myocardial infarction, incidence of cardiac arrhythmia and serum MDA yet increased activity of SOD and GSH-PX. However, the difference in groups LPC and RLPC did not reach statistical significance （all P〉0.05）. Conclusion The protective effects of RLPC on rat cardiac ischemia-reperfusion injury were as powerful as LPC.