| bcl-2硫代反义寡核苷酸提高胃癌细胞凋亡的敏感性 |
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| 引用本文： | Cao J,Qiao Y,Min J. bcl-2硫代反义寡核苷酸提高胃癌细胞凋亡的敏感性[J]. 中华肿瘤杂志, 2000, 22(6): 466-468 |
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| 作者姓名： | Cao J Qiao Y Min J |
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| 关 键 词： | 胃肿瘤 bcl2基因 反义寡核苷酸 细胞凋亡 |
Bcl-2 anti-sense oligonucleotide sensitizes Fas-mediated apoptosis of gastric cancer cells |
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| Cao J,Qiao Y,Min J. Bcl-2 anti-sense oligonucleotide sensitizes Fas-mediated apoptosis of gastric cancer cells[J]. Chinese Journal of Oncology, 2000, 22(6): 466-468 |
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| Authors: | Cao J Qiao Y Min J |
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| Affiliation: | Department of Surgery, People's Hospital of Taishan, Taishan 529200, China. |
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| Abstract: | OBJECTIVE: To study the effect of bcl-2 anti-sense oligonucleotide on the sensitivity of gastric cancer cells to Fas-mediated apoptosis. METHODS: Gastric cancer cell line MKN45 was transfected with bcl-2 anti-sense oligonucleotide, and expression of bcl-2 was examined by Western blotting. Agonistic Fas antibody was used to induce apoptosis detected by TUNEL staining and flow cytometry. RESULTS: Bcl-2 expression in MKN45 cells transfected with bcl-2 anti-sense oligonucleotide was markedly inhibited. When cultured with antibody apoptosis index of the anti-sense oligonucleotide-treated MKN45 cells was 55.6% +/- 4.7% (n = 5), which was significantly higher than that of the control (8.4% +/- 2.1%, n = 5). CONCLUSION: Expression of bcl-2 in gastric cancer cells may antagonize Fas-mediated apoptosis. |
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