多形性胶质母细胞瘤放疗前后CXCL14和IGLV1-44的表达及其临床意义

目的探讨多形性胶质母细胞瘤(GBM)组织照射60Gy后基因表达谱中免疫相关基因表达差异的变化及意义。方法采用BioStarH-141S(2004)型含13 929条人类全长基因cDNA表达谱芯片,对2例多形性胶质母细胞瘤组织进行放疗前及放疗60Gy后检测,并分析它们之间的基因表达差异。结果多形性胶质母细胞瘤放疗60Gy后与放疗前比较,表达差异基因中改变最明显的功能群是免疫系统相关的基因,如CXCL14和IGLV1-44上调。细胞增殖、细胞凋亡、细胞周期、DNA修复系统也有部分基因发生明显变化,如MLL5上调、POLR2B下调等。结论提示对多形性胶质母细胞瘤组织照射60Gy后基因表达谱改变的研究可以更好地阐明放射敏感性差异机制,为放疗前或放疗早期寻找到预测放射敏感性分子标志提供理论依据。

Expression profile of glioblastoma tissue CXCL14 and IGLV1-44 before-and-after the radiotherapy and its significance

Objective To study the change of gene express profile of glioblastoma(GBM) tissue before-and-after the radiation of 60Gy and its significance.Methods BioStarH-141S(2004) profile genechip which contained 13 929 points of full length human genes was used to detect two GBM tissue before-and-after radiation and to analyze the genes expressed differently.Results The results showed that,after radiation of 60Gy,the gene group which changed the most obviously was related to the function of immunity,for instance,up-regulate genes: CXCL14,IGLV1-44.Some genes related to cell proliferation,apoptosis,cell cycle and DNA-repair system,also differently expressed in evidence,for instance,up-regulate gene: MLL5 and down-regulate gene: POLR2B.Conclusion The results indicated that,research the change of the gene express profile of GBM tissue before-and-after the radiation could better clarify the mechanism of radiation sensitivity.It may afford theoretic idea to find molecular markers to predict radiation sensitivity before or at the forepart of radiation therapy.