Visualization of hepatobiliary excretory function by intravital multiphoton microscopy |
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Authors: | Liu Yuan Chen Hsiao-Ching Yang Shu-Mei Sun Tzu-Lin Lo Wen Chiou Ling-Ling Huang Guan Tarn Dong Chen-Yuan Lee Hsuan-Shu |
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Affiliation: | National Taiwan University, Department of Physics, Taipei 106, Taiwan. |
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Abstract: | Intravital imaging of hepatobiliary excretion is vital for elucidating liver metabolism. In this work, we describe a novel method to observe the intravital dynamics of the uptake, processing, and excretion of an organic anion, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary system. This is achieved by the use of multiphoton microscopy and an intravital hepatic imaging chamber. The high-quality images show sequential uptake and processing of 6-CFDA from the hepatocytes and the subsequent excretion into bile canaliculi within approximately 50 min. This is a promising technique to study intravital hepatic physiology and metabolism. |
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