血脂康对动脉粥样硬化的预防作用

目的:探讨血脂康对斑马鱼动脉粥样硬化的预防作用。方法:以二甲基亚砜为阴性对照,辛伐他汀为阳性对照,对斑马鱼脂代谢紊乱模型进行不同浓度的血脂康处理,通过油红O染色、直接测量血脂、荧光显微镜拍照等方法评估血脂康对动脉粥样硬化的预防作用。结果:油红O染色显示血脂康10μg/mL及以上浓度处理组中幼鱼血管内脂质沉积显著减轻;脂质含量测定显示血脂康5μg/mL处理组幼鱼组织匀浆中各类脂质成分明显降低;荧光显微镜活体观察发现血脂康2μg/mL及以上浓度处理组幼鱼的中性粒细胞在血管内的募集受到显著抑制。结论:血脂康通过降低血脂和抑制中性粒细胞在血管内的募集,预防动脉粥样硬化。

Preventive effects of Xuezhikang on atherosclerosis

Objective:To explore the preventive effects of Xuezhikang on atherosclerosis in zebrafish.Methods:Using dimethyl sulfoxide as the negative control and simvastatin as the positive control,the zebrafish models of lipid metabolism disorder were exposed in different concentrations of Xuezhikang.Oil red O staining,direct measurement of lipid content and fluorescence microscopy were used to evaluate the preventive effects of Xuezhikang on atherosclerosis.Results:Oil Red O staining showed significant reduction in intravascular lipid deposition in zebrafish of Xuezhikang groups,which were exposed in 10μg/mL and above Xuezhikang.Lipid measurements revealed that various types of lipids in whole tissue homogenate of zebrafish in 5μg/mL Xuezhikang treatment group were significantly decreased.In vivo observation with the fluorescent microscope displayed that neutrophil recruitment in zebrafish of the 2μg/mL and above Xuezhikang treatment groups was significantly inhibited.Conclusions:The prevention of atherosclerosis by Xuezhikang may be caused by lowering blood lipids and inhibiting the recruitment of neutrophils in blood vessels.