藤茶双氢杨梅树皮素联合环磷酰胺抗肿瘤作用研究

目的:探讨藤茶双氢杨梅树皮素(APS)联合环磷酰胺(CTX)抗肿瘤的作用。方法:将接种H22的小鼠按瘤体积大小随机分为6组:模型对照组、环磷酰胺组、APS高剂量组、APS低剂量+环磷酰胺组、APS中剂量+环磷酰胺组、APS高剂量+环磷酰胺组,每组14只。环磷酰胺组予环磷酰胺溶液灌胃,剂量为0.02 g/kg;APS高剂量组予双氢杨梅树皮素灌胃,剂量为0.60 g/kg;APS低剂量+环磷酰胺组:灌胃双氢杨梅树皮素0.15 g/kg+腹腔注射环磷酰胺0.02 g/kg;APS中剂量+环磷酰胺组:灌胃双氢杨梅树皮素0.30 g/kg+腹腔注射环磷酰胺0.02 g/kg;APS高剂量+环磷酰胺组:灌胃双氢杨梅树皮素0.60 g/kg+腹腔注射环磷酰胺0.02 g/kg;各组灌胃容积均为10 ml/kg。给药10 d后,计算小鼠的抑瘤率、脾指数、胸腺指数、肝脏指数,分析APS联合环磷酰胺抗肿瘤的作用。结果:APS可抑制H22荷瘤小鼠肿瘤的生长,抑瘤率为20.72%;单用CTX组抑瘤率为33.5%,APS低、中、高剂量与CTX联用后抑瘤率分别为47.07%、48.52%和60.26%;与单用CTX组比较,联合用药明显提高了对H22荷瘤小鼠的抑瘤作用(P<0.05)。与模型对照组比较,CTX组脾指数和胸腺指数明显下降(P<0.01);与CTX组比较,APS中、高剂量与CTX联合用药后可提高小鼠的脾指数和胸腺指数,差异有统计学意义(P<0.05或P<0.01);APS低剂量与CTX联合用药后可明显提高小鼠的胸腺指数(P<0.01);APS高剂量与CTX联合用药后还可明显提高小鼠的肝脏指数(P<0.01)。结论:APS对H22肿瘤具有抑制作用,与环磷酰胺联用能增强环磷酰胺对H22荷瘤小鼠的抗肿瘤作用。

Study on Synergistic Effect of Ampelopsin combined with Cyclophosphamide in Anti-Tumor Therapy

Objective:To investigate the synergistic effect of ampelopsin(APS)combined with cyclophosphamide in anti-tumor therapy.Methods:According to the size of tumor the H22-bearing mice were randomly divided into 6 groups:model control group,cyclophosphamide group,APS high-dose group,and low-,medium-and high-does of APS combined separately with CTX groups,with 14 mice in one group.In the cyclophosphamide group intragastric administation of cyclophosphamide solution at a dose of 0.02 g/kg was given;in the APS high-dose group intragastric administration of dihydromyricetin with a dose of 0.60 g/kg was given;APS low-dose pluscyclophosphamide group:intragastric administration of dihydromyricetin 0.15 g/kg plusintraperitoneal injection of cyclophosphamide 0.02 g/kg were given;APS medium-dose plus cyclophosphamide group:intragastric administration of dihydromyricetin 0.30 g/kg plusintraperitoneal injection of cyclophosphamide 0.02 g/kg were given;APS high-dose plus cyclophosphamide group:intragastric administration of dihydromyricetin 0.60 g/kg plusintraperitoneal injection of cyclophosphamide 0.02 g/kg;the volume of intragastric administration in each group was 10 ml/kg.After 10 days of treatments,the inhibition rate,spleen index,thymus index,liver index were calculated,and the synergistic effect of APS on CTX was analyzed.Results:APS inhibited the growth of H22 tumor-bearing mice.The tumor inhibition rate of APS was 20.72%which were higher than that of CTX administration alone(33.5%),The tumor inhibition rate of the low,medium and high dose groups of APS combined separately with CTX was 47.07%,48.52%and 60.26%.Compared with CTX group,APS combined with CTX significantly improved the inhibition of H22 tumor-bearing mice(P<0.05).Compared with the model control group,the spleen index and thymus index of CTX group significantly decreased(P<0.01).The medium and high dose groups of APS combined with CTX can significantly increase the spleen index,thymus index(P<0.05 or P<0.01).And the high dose of APS can significantly increase the live index(P<0.01).Conclusion:APS can inhibit the tumor of H22 and promote the anti-tumor effect of CTX on H22 tumor-bearing mice in the combined application.