| 二步温控法聚合酶链反应检测脑脊液结核杆菌DNA |
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| 引用本文： | 文芳,刘学锋,王镇涛,万均成,林琼. 二步温控法聚合酶链反应检测脑脊液结核杆菌DNA[J]. 中华检验医学杂志, 1994, 0(5) |
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| 作者姓名： | 文芳 刘学锋 王镇涛 万均成 林琼 |
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| 作者单位： | 湖北医学院附属第一医院 |
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| 摘 要： | 应用二步温控法聚合酶链反应（ＰＣＲ）检测脑脊液结核杆菌微量ＤＮＡ，并扩增出标准人型结核杆菌１５８ｂｐ的基因片段。实验结果表明：ＰＣＲ用于结核性脑膜炎患者脑脊液结核杆菌ＤＮＡ的检测，与传统检验方法相比较，具有敏感、快速、特异、高效等优点，是基因水平上检测结核杆菌的一项新技术，用于结核性脑膜炎的早期诊断，具有很强的实用价值，值得临床推广应用。
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| 关 键 词： | 结核杆菌；结核；脑膜；脑脊髓液；聚合酶链反应 |
Two-step protocol polymerase chain reaction for detection of mycobacterium tuberculosis DNA in thecerebrospinal fluid |
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| Wen Fang, Liu Xue feng, Wang Zhentao,et al.. Two-step protocol polymerase chain reaction for detection of mycobacterium tuberculosis DNA in thecerebrospinal fluid[J]. Chinese Journal of Laboratory Medicine, 1994, 0(5) |
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| Authors: | Wen Fang Liu Xue feng Wang Zhentao et al. |
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| Affiliation: | Wen Fang, Liu Xue feng, Wang Zhentao, et al. |
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| Abstract: | he two-step protocol polymerase chain reaction(PCR)was used to detect microquantity DNA ofmycobacterium tuberculosis in the cerebrospinal fluid. 158bp DNA fragment of standard humanmycobacterium tuberculosis were amplified. The result suggested that the method was more sensitive,quicker, and more spcific in comparison with conventional experiment methods. It is a new techniquefor detection of mycobacterium tuberculosis from gene and is of important value for early diagnosis oftuberculous meningitis.It deserves clinical application in a wide scale. |
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| Keywords: | Mycobacterium tuberculosis Tuberculosis meningeal Cerebrospinal fluid Polymerase chain reaction |
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