5-FU耐药免疫组化技术与MTT法敏感性比较

目的:探讨结肠癌对5-氟脲嘧啶(5-FU)产生耐药的机理,从细胞培养水平进一步论证胸苷酸合成酶(TS)对5-FU化疗产生耐药的影响,揭示不同TS蛋白表达水平的结肠癌患者对5-FU治疗的敏感性。方法:应用MTT法对体外培养的30例结肠癌细胞进行5-FU药敏试验,再应用SP免疫组化法对30例结肠癌进行TS检测,然后将两个试验结果进行比较;另采用SP法检测60例结肠癌组织切片及其正常对照组切片中TS蛋白的表达情况。结果:TS高表达组体外培养的结肠癌细胞对5-FU的敏感性明显低于TS低表达组。正常结肠粘膜组织中的TS表达水平高于结肠癌组织,结肠癌组织中TS表达水平与5-FU化疗患者的预后呈负相关,与患者的性别、有无淋巴结转移及癌组织的分化程度无关。结论:TS蛋白表达程度的高低对临床结肠癌患者化疗药物的选择有一定的指导意义;结肠癌组织中TS蛋白的表达水平可作为患者对5-FU为主的化疗疗效及预后的判断指标。

The Comparison between 5-FU Drug Resistance Efficacy s-p Immunohistochemical Staining and the MTT Method

Objective: To investigate the reason of the 5-FU drug resistance, and to prove deeply the influence of TS in colon carcinoma with the level of cell culture. and to evaluate the expression of TS could be as a chemotherapeutic efficacy index in patients with colon carcinoma. Methods: To proceed the 5-FU drug susceptibility test with MTT method to 30 cases in vitro culture colon carcinoma cell; to be examined by SP immunohistochemical staining and observed the expression of TS; and then compare with them. The expression of TS in 60 cases with colon carcinoma and their control cases were examined by SP immunohistochemical staining. Results: In vitro culture colon carcinoma cells corresponding high-expression of TS the sensibity to 5-FU delay obviously than the low-expression. The level of TS in normal colon tissues was higher than that in colon carcinoma. The level of TS in well-differentiated colon carcinoma was higher than that in poor-differentiated cases. There was an inverse association between the level of colon carcinoma TS expression and clinical prognosis, the level of TS showed no correlation with sex and lymph node metastases. Conclusions: The positive expression of TS has a instruction significance in colon carcinoma patients at the election of chemistry drug, it can be as a 5-FU based chemotherapeutic efficacy and clinical prognosis index in patients with colon carcinoma.