抑肽酶对大鼠实验性慢性肝损伤的治疗作用

目的:研究抑肽酶对大鼠慢性肝损伤的治疗作用。方法: Wistar大鼠60只，随机分为6组(每组10只)：第Ⅰ组为正常对照组；第Ⅱ～Ⅵ组采用皮下注射四氯化碳（CCl₄）12周建立慢性肝损伤实验动物模型后，进行不同处置，即第Ⅱ组为模型组、第Ⅲ组为抑肽酶小剂量（2万kIU?kg^-1）组、第Ⅳ组为抑肽酶中剂量（4万kIU?kg^-1）组、第Ⅴ组为抑肽酶大剂量（8万kIU?kg^-1）组、第Ⅵ组为阳性对照促肝细胞生长素（100 mg?kg^-1）组。第8周后第Ⅲ～Ⅵ组分别用抑肽酶和肝细胞生长素每日腹腔注射1次，连续4周后测定各组肝损伤大鼠血清丙氨酸氨基转换酶（ALT）、天门冬氨酸氨基转换酶(AST)、白蛋白(ALB)、总蛋白(TP)、唾液酸（SA）活性及肝组织羟脯氨酸（Hyp）含量，观察光镜下肝组织的病理改变。结果:模型组与正常对照组比较，血清ALT、AST活性和肝组织Hyp显著升高，血清SA、ALB和A/G比值显著降低。抑肽酶各组与模型组比较，血清ALT、AST活性及Hyp含量降低，血清SA、ALB和A/G比值明显增高。肝组织病理切片观察显示，抑肽酶能显著减轻由CCl₄所致肝细胞脂肪变性及纤维组织增生。结论：抑肽酶对大鼠慢性肝损伤具有明显的治疗作用。

Therapeutic effects of aprotinin on experimental chronic hepatic injury in rats

Objective To study the therapeutic effects of aprotinin on chronic hepatic injury in rats. Methods Sixty Wistar rats were divided into 6 groups(n=10). Group Ⅰ: normal control group, Group Ⅱ-Ⅵ: the chronic hepatic fibrosis models of rats were induced by carbon tetrachloride (CCl_4) for 12 weeks, Group Ⅱ: model control group, Group Ⅲ: low dose of aprotinin (2×10~4 kIU·kg~ -1 )group, Group Ⅳ:middle dose of aprotinin (4×10~4 KIU·kg~ -1 ) group, Group Ⅴ: high dose of aprotinin (8×10~4 KIU·kg~ -1 )group, Group Ⅵ: pHGF (100 mg·kg~ -1 ) control group. After 8 weeks in GroupⅢ-Ⅵ,per rat was injected with aprotinin and pHGF every day respectively for 4 weeks. The changes of activiies of serum ALT,AST,TP,ALB,A/G,SA and Hyp content of liver tissue were measured. The pathologic changes of liver tissue were observed.Results Compared with normol control group, The activities of serum ALT,AST and the Hyp level of liver tissue were increased;the activities of serum SA,ALB and A/G were decreased in model group. Compared with model group,aprotinin could decrease the activities of serum ALT, AST and Hyp level of liver tissue,increase the activities of serum SA,ALB and A/G. Hepatic histological findings indicated that aprotinin could alleviate chronic hepatic injury significantly. Conclusion Aprotinin has therapeutic functions on chronic hepatic injury in rats.