输精管结扎对大鼠睾丸生精细胞凋亡的影响

为探讨输精管结扎对睾丸曲细精管生精细胞凋亡程度的影响，应用流式细胞仪和脱氧核糖核酸末端转移酶介导的ｄＵＴＰ缺口末端标记（ＴＵＮＥＬ）技术，检测大鼠输精管结扎和假手术后２～１２周睾丸生精细胞的凋亡情况。结果：流式细胞仪检测发现结扎组的亚单倍体峰增高，亚单倍体细胞百分率升高；ＴＵＮＥＬ法则显示结扎后总的生精细胞凋亡数较假手术后明显增加（Ｐ＜０．００１）。结扎后不同时间组与同期假手术组比较，２周、６周、１０周组精原细胞和初级精母细胞凋亡数前者均高于后者（Ｐ均＜０．０５），１２周组精原细胞凋亡数前者高于后者（Ｐ＜０．０５）。结果表明输精管结扎会导致生精细胞凋亡增加。

Effect of Vasectomy on Apoptosis in Spermatogenic Cells of the Male Rat

To explore the effect of vasectomy on apoptosis of spermatogenic cells, we have detected apoptosis in the testis of vasectomized and sham operated adult rats within a period of 12 postoperative weeks by means of flow cytometry and TUNEL (terminal deoxynucleotidyl transferase mediated deoxyuride triphosphate nick end labeling) method. Flow cytometry found that, in vasectomized rat, the sub haploid peak was higher and the percentage of cells with sub haploid was increased. The total number of positive TUNEL cells in the vasectomized group was greater than that in the sham operated group ( P<0.001 ). Compared with the same time phased sham operated subgroups, the number of apoptotic spermatogonia and primary spermatocytes in the testes of the subgroups 2 weeks, 6 weeks and 10 weeks after vasectomy as well as the number of spermatogonia of the subgroup 12 weeks after vasectomy were sigificantly higher ( P<0.05) . These findings demonstrate that vasectomy could enhance the apoptosis of spermatogenic cells.