脂肪干细胞源性外泌体对瘢痕成纤维细胞生物学活性的影响

目的 观察脂肪干细胞源性外泌体（ADSC-Exos）对瘢痕成纤维细胞生物学活性的调控作用。方法 将瘢痕成纤维细胞分为对照组、ADSC-Exos组。采用CCK-8法检测不同组别中瘢痕成纤维细胞的增殖情况；采用蛋白免疫印迹法检测纤维化指标的表达量；采用实时荧光定量-PCR检测纤维化相关基因mRNA的表达水平。结果 与对照组相比，在ADSC-Exos作用下，瘢痕成纤维细胞的增殖能力减弱，纤维化相关指标水平降低（P均<0.05）。瘢痕成纤维细胞在ADSC-Exos的作用下，Ⅰ、Ⅲ胶原及α-平滑肌肌动蛋白的mRNA表达下调（P均<0.05）。结论 ADSCExos能抑制瘢痕成纤维细胞的生物学活性。

Effect of exosomes from adipose-derived stem cells on the biological activity of scar fibroblasts

Objective To observe the regulatory effect of exosomes from adipose-derived stem cells （ADSC-Exos） on the biological activity of scar fibroblasts. Methods Scar fibroblast were prepared and divided into the control and ADSC-Exos groups. The proliferation of scar fibroblasts between two groups was observed by CCK-8 assay. The expression levels of fibrosis-related indexes were detected by Western blot. The expression levels of fibrosis-related gene mRNA were determined by fluorescent quantitative real-time （RT） -PCR. Results Compared with the control group, the proliferation capability of scar fibroblasts was significantly weakened, and the fibrosis-related indexes were significantly decreased in ADSC-Exos group （both P < 0.05）. Meanwhile, the expression levels of collagen I, III and α-smooth muscle actin mRNA of scar fibroblasts were significantly down-regulated in the ADSC-Exos group（all P < 0.05）. Conclusion ADSC-Exos can inhibit the biological activity of scar fibroblasts.