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卵巢癌组织中DR-NM23的表达及对细胞增殖和转移的影响
引用本文:周 红,周江洪. 卵巢癌组织中DR-NM23的表达及对细胞增殖和转移的影响[J]. 现代检验医学杂志, 2022, 0(1): 114-118. DOI: 10.3969/j.issn.1671-7414.2022.01.024
作者姓名:周 红  周江洪
作者单位:(中南大学湘雅医学院附属株洲医院妇科,湖南株洲 412000)
摘    要:目的 探究DR-NM23在卵巢癌组织中的表达及对细胞增殖和转移的影响。方法 收集卵巢癌肿瘤组织和癌旁组织各68例,通过免疫组化染色检测DR-NM23表达水平,分析绝经、肿瘤大小、分化程度、局部浸润、淋巴转移和远端转移与DR-NM23表达水平的相关性。在SKOV3细胞系中过表达、沉默DR-NM23,分别得到DR-NM23组和SiDRNM23组,与对照组(NC组)相比,评估各组增殖、迁移和侵袭能力,以评估DR-NM23在卵巢癌组织中的作用机制。结果 卵巢癌组织中DR-NM23平均染色强度为2.68±0.75,低于癌旁组织7.05±2.05,差异具有统计学意义(P<0.05)。卵巢癌组织中DR-NM23表达水平与是否绝经、肿瘤大小以及局部浸润无相关性,差异均无统计学意义(χ2=0.418, 0.864,2.915,均P>0.05)。低分化、出现淋巴转移和远端转移的组织中DR-NM23表达水平相对较低,差异均具有统计学意义(χ2=6.678, 6.845, 8.215,均P<0.05)。SiDR-NM23组细胞的CCK-8吸光度(1...

关 键 词:卵巢癌  DR-NM23  增殖  迁移  侵袭

Expression of DR-NM23 in Ovarian Cancer Tissue and Its Effect on Cell Proliferation and Metastasisy
ZHOU Hong,ZHOU Jiang-hong. Expression of DR-NM23 in Ovarian Cancer Tissue and Its Effect on Cell Proliferation and Metastasisy[J]. Journal of Modern Laboratory Medicine, 2022, 0(1): 114-118. DOI: 10.3969/j.issn.1671-7414.2022.01.024
Authors:ZHOU Hong  ZHOU Jiang-hong
Affiliation:(Department of Gynaecology, Zhuzhou Hospital Affiliated to Xiangya MedicalCollege of Central South University, Hunan Zhuzhou 412000, China)
Abstract:Objective To explore the expression of DR-NM23 in ovarian cancer and its effect on cell proliferation andmetastasis. Methods 68 cases of ovarian cancer tissues and adjacent tissues were collected clinically, and the DR-NM23expression level was detected by immunohistochemistry, and the correlation of DR-NM23 expression level with menopause,tumor size, differentiation, local invasion, lymph node metastasis and distal metastasis was analyzed. Overexpression andsilencing of DR-NM23 in SKOV3 cell line were used to obtain DR-NM23 group and Si-NM23 group, respectively. Comparedwith the control group (NC group), the proliferation, migration and invasion ability of each group were evaluated for themechanism of DR-NM23 in ovarian cancer tissue. Results The average staining intensity of DR-NM23 in ovarian cancer tissuewas (2.68±0.75), which was lower than that in adjacent tissues (7.05±2.05) with stastically significant difference (P<0.05).Whether there was no correlation in menopause, tumor size and local invasion with the expression level of DR-NM23 in ovariancancer tissue, the differences not were statistically significant(χ2=0.418, 0.864, 2.915, all P>0.05). The expression level ofDR-NM23 was lower in tissues with poor differentiation, lymph node metastasis and distant metastasis, the differences werestatistically significant (χ2=6.678, 6.845, 8.215, all P<0.05). The CCK-8 absorbance (1.16±0.18), migration distance percentage(88.58%±5.48%) and invasive cells (207.26±13.52) of siDR-NM23 group was higher than that of NC group(0.68±0.11,62.34%±2.15%, 125.54±8.15), while DR-NM23 group(0.47±0.07, 45.12%±3.76%, 59.57±5.02) was lower than that of NCgroup, the differences were stastically significant (all P<0.05). Conclusion DR-NM23 was lowly expressed in ovarian cancertissue, might has the effect of inhibiting the proliferation, migration and invasion of tumor cells.
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