4-1BB、CD8在口腔扁平苔藓组织中的表达研究

目的 检测共刺激信号4-1BB及CD8在口腔扁平苔藓(oral lichen planus,OLP)组织中的表达,探讨OLP病损中4-1BB介导CD8⁺ T细胞免疫应答的潜在机制。方法 选取31例OLP患者(糜烂型15例、非糜烂型16例),15例健康成人(对照组)为研究对象,收集其临床及病理资料,采用免疫组织化学法检测局部组织中4-1BB、CD8的表达情况。结果 OLP患者局部组织中4-1BB及CD8的表达高于对照组(P<0.05),且两者表达呈正相关(r=0.389,P<0.05),糜烂型OLP患者中CD8、4-1BB表达高于非糜烂型(P<0.05)。在OLP不同的临床亚组中,VAS评分、REU评分及基底细胞损伤程度均表现出差异(P<0.05)。Spearman相关性分析结果显示,CD8与基底细胞损伤程度呈正比(P<0.05),CD8、4-1BB分别与VAS评分、REU评分存在正相关性(P<0.05)。结论 OLP患者局部组织中4-1BB表达升高,其与疾病活动进展程度呈正相关,4-1BB可能通过调控CD8⁺ T细胞参与OLP的发生发展。

Correlation between 4-1BB and CD8 expression in oral lichen planus

Objective To examine the expression of 4-1BB and CD8 in oral lichen planus (OLP) tissues and to investigate the potential mechanism of 4-1BB on CD8⁺ T cell-mediated immune response in OLP. Methods Thirty-one OLP cases(15 erosive and 16 non-erosive) and 15 healthy adults (control group) were selected as study subjects and their clinical pathological data were collected.Immunohistochemistry was used to detect the expression of 4-1BB and CD8. Results The expression of 4-1BB and CD8 in local tissues of OLP were higher than those in the control group(P<0.05), and the expression of 4-1BB and CD8 in OLP were positively correlated(r=0.389,P<0.05). Besides, the expression of 4-1BB and CD8 in erosive OLP were higher than those in non-erosive OLP(P<0.05).There was a difference between OLP subgroups in terms of VAS score, REU score and the degree of basal cell injury (P<0.05). Spearman correlation analysis showed that CD8 was positively correlated with the degree of basal cell injury (P<0.05), and CD8 and 4-1BB were positively correlated with VAS score and REU score(P<0.05). Conclusion There is an abnormal increase of 4-1BB in OLP, and the increase is positively correlated with disease activity progression, suggesting that 4-1BB may be involved in the development of OLP by regulating CD8⁺ T cells.