人脐带和胎盘间充质干细胞细胞因子分泌的比较研究

目的 探索人脐带间充质干细胞（UC-MSC）与胎盘间充质干细胞（P-MSC）细胞因子的分泌情况。方法 分离并培养UC-MSC和P-MSC，流式细胞术检测间充质干细胞（MSC）的表面标志物，油红O、硝酸银和茜素红S染色检测MSC的成脂、成骨分化能力。收集条件培养基，用芯片检测细胞因子的表达情况。通过ELISA及实时PCR...

Comparison of cytokine profile in mesenchymal stem cells derived from human umbilical cord and placenta

Objective To compare the secretion of cytokines from human umbilical cord-derived mesenchymal stem cells （UC-MSCs） and placenta-derived mesenchymal stem cells （P-MSCs）. Methods UC-MSCs and P-MSCs were isolated and cultured. Cell surface antigens of MSCs were measured using flow cytometry. The adipogenic and osteogenic differentiation capability was analyzed by Oil red O, Von Kossa staining and Alizarin red S staining. The conditioned medium was collected and the expression of cytokines was detected by microarray. Microarray assay results were validated by ELISA and RT-PCR. Results UC-MSCs and P-MSCs had similar surface markers,adipogenic and osteogenic differentiation capacity. Both UC-MSCs and P-MSCs highly expressed insulin-like growth factor-binding protein （IGFBP）-3, IGFBP-4 and IGFBP-6, tissue inhibitor of metallo proteinase （TIMP）-1 and TIMP-2, IL-6, TGF-β1, hepatocyte growth factor, monocyte chemoattractant protein-1 and human intercellular adhesion molecule-1, whereas lowly expressed epidermal growth factor, IL-17, IL-12p40,B lymphocyte chemoattractant, tumor necrosis factor-β （TNF-β）, IL-13, MIP-1δ, Eotaxin-2, IL-12p70 and platelet-derived growth factor-BB. Compared with UC-MSCs,P-MSCscontained five differentially-expressed proteins including TNF receptorⅡ, stem cell factor, nerve growth factor receptor, TGF-β3 and IL-1β. The results of ELISA and RT-PCR were consistent with themicroarray protein data. Conclusion UC-MSCs and P-MSCs have similar surface markers, osteogenic and adipogenic differentiation capacity and cytokine profile.