miRNA-34b 对子宫内膜癌细胞凋亡、侵袭、迁移的影响及作用机制

目的 探究miR-34b 对子宫内膜癌(endometrial cancer) 凋亡、迁移、侵袭的影响及对Jag1/Cyclin D1 信号通路的影响。方法 将人子宫内膜癌细胞AN3CA 细胞分为对照组、NC 组和miR-34b 组。通过流式细胞技术、Transwell实验分别检测各组细胞凋亡、迁移能力和侵袭能力；通过定量聚合酶链反应(quantitative polymerase chain reaction，qPCR)及Westernblot 检测各组细胞Jagged-1 蛋白(Jag1)及其靶基因G1/S- 特异性周期蛋白-D1(Cycling D1)mRNA和蛋白的表达水平；通过裸鼠荷瘤实验分析miR-34b 对肿瘤生长的影响。结果 对照组、NC 组和miR-34b 组细胞凋亡比例分别为5.6%±0.12%，5.80%±0.22% 和19.4%±0.51%。miR-34 组细胞凋亡比例高于对照组，差异具有统计学意义(t=8.325，P ＜ 0.01)；对照组、NC 组和miR-34b 组细胞迁移数分别为322.15±13.71 个，327.67±9.14 个和162.19±7.49 个，miR-34b 组细胞迁移数低于对照组，差异具有统计学意义(t=7.945 , P ＜ 0.01)；对照组、NC 组和miR-34b 组细胞侵袭数分别为357.6±14.11 个，364.05±16.12 个和157.58±8.77 个，miR-34b 组细胞侵袭数低于对照组，差异具有统计学意义(t=7.643，P ＜ 0.01)；qPCR 结果表明对照组、NC 组和miR-34b 组Jag1 mRNA 表达为2.75±0.21，2.67±0.14 和1.19±0.19，miR-34b 组低于对照组，差异具有统计学意义(t=8.434，P ＜ 0.01)；对照组、NC 组和miR-34b 组蛋白表达为1.71±0.11，1.77±0.24 和0.69±0.16，miR-34b 组低于对照组，差异具有统计学意义(t=7.895，P ＜ 0.01)；对照组、NC 组和miR-34b 组Cyclin D1mRNA 表达为1.29±0.13，1.32±0.11 和0.59±0.13，miR-34b 组低于对照组，差异具有统计学意义(t=9.124，P ＜ 0.01)；对照组、NC 组和miR-34b 组蛋白表达为1.81±0.18，1.79±0.14和1.29±0.16，miR-34b 组低于对照组，差异具有统计学意义(t=8.227，P ＜ 0.01)；裸鼠荷瘤实验表明过表达miR-34b 裸鼠肿瘤生长曲线与对照组差异有统计学显著性意义，并且肿瘤体积显著低于对照组(t=8.184，P ＜ 0.01)。结论 miR-34b 可以促进人子宫内膜癌细胞的凋亡，抑制细胞迁移和侵袭能力，其机制与Jag1/Cyclin D1 信号通路活性的抑制相关。

Effect and Mechanism of miR-34b on Apoptosis,Invasion and Migration of Endometrial Cancer Cells

Objective To investigate the effect and mechanism of miR-34b on apoptosis, migration and invasion of endometrial cancer cells. Methods Human endometrial cancer cell line AN3CA was divided into control group, NC group and miR-34b group. The apoptosis, migration and invasion of each group were detected by flow cytometry and Transwell experiment respectively. The mRNA and protein expression levels of Notch ligand (Jag1) and its target gene G1/S-specific cyclin-D1 were detected by qPCR and Western blot. The effect of miR-34b on tumor growth was analyzed by tumor bearing experiment in nude mice. Results The apoptosis rates of control group, NC group and miR-34b group were 5.6%±0.12%, 5.80%±0.22% and 19.4%±0.51%, respectively. The proportion of apoptosis in miR-34 group was higher than that in control group, the difference was statistically significant (t = 8.325, P ＜ 0.01). The number of cell migration in the control group, NC group and miR-34b group were 322.15±13.71, 327.67±9.14 and 162.19±7.49, respectively. The number of cell migration in miR-34b group was lower than that in the control group, and the difference was statistically significant (t = 7.945, P ＜ 0.01). The number of cell invasion in the light group, NC group and miR-34b group were 357.6 ± 14.11,364.05±16.12 and157.58±8.77, respectively, and the number of cell invasion in miR-34b group was lower than that in the control group, and the difference was statistically significant (t =7.643, P ＜ 0.01). QPCR results showed that the expression of Jag1 mRNA in control group, NC group and miR- 34b group was 2.75±0.21, 2.67±0.14 and 1.19±0.19, respectively. The expression of Jag1 mRNA in miR-34b group was lower than that in control group, and the difference was statistically significant (t = 8.434, P ＜ 0.01). The protein expression in control group, NC group and miR-34b group was 1.71±0.11, 1.77±0.24 and 0.69±0.16, respectively. The expression of Jag1 mRNA in miR-34b group was lower than that in control group, and the difference was statistically significant (t = 7.895, P ＜ 0.01). The expression of cyclin D1 mRNA in control group, NC group and miR-34b group was 1.29±0.13, 1.32±0.11 and 0.59±0.13, respectively. The expression of cyclin D1 mRNA in miR-34b group was lower than that in control group, and the difference was statistically significant (t= 9.124, P ＜ 0.01). The protein expression in control group, NC group and miR-34b group was 1.81±0.18, 1.79±0.14 and 1.29±0.16, respectively. The expression of cyclin D1 mRNA in miR-34b group was lower than that in control group, the difference was statistically significant (t= 8.227, P ＜ 0.01). The tumor bearing experiment in nude mice showed that the tumor growth curve of overexpressing miR-34b nude mice was significantly different from that of the control group, and the tumor volume was significantly lower than that of the control group (t = 8.184, P ＜ 0.01). Conclusion MiR-34b can promote apoptosis, inhibit cell migration and invasion of human endometrial cancer cells, and it is related to the inhibition of Jag1/cyclin D1 signal pathway activity.